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Literature summary extracted from

  • Martinou, A.; Koutsioulis, D.; Bouriotis, V.
    Expression, purification, and characterization of a cobalt-activated chitin deacetylase (Cda2p) from Saccharomyces cerevisiae (2002), Protein Expr. Purif., 24, 111-116.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.41 acetate 50 mM, 50% loss of activity Saccharomyces cerevisiae
3.5.1.41 Ca2+ 1-100 mM Saccharomyces cerevisiae
3.5.1.41 Cu2+ 1-100 mM Saccharomyces cerevisiae
3.5.1.41 EDTA
-
 Saccharomyces cerevisiae
3.5.1.41 Mg2+ 1-100 mM Saccharomyces cerevisiae
3.5.1.41 additional information N-deglycosylation of the enzyme causes total loss of activity, which can be restored by addition of 1 mM CoCl2 Saccharomyces cerevisiae
3.5.1.41 Zn2+ 1-100 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.41 1.6
-
 (GlcNAc)4 pH 8.0, 50°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.41 Co2+ increases activity from 1-50 mM. 160% relative activity at 1 mM. Deglycosylation of the enzyme causes total loss of activity, which can be restored by addition of CoCl2 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.41 43000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.41 glycoprotein contains 18% carbohydrate by weight. Only N-linked glycans exist Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41
-
 Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.41 771
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O 39% deacetylation Saccharomyces cerevisiae ?
-
 ?
3.5.1.41 N,N',N'',N'''-tetraacetylchitotetraose + H2O 22% deacetylation Saccharomyces cerevisiae ?
-
 ?
3.5.1.41 N,N',N''-triacetylchitotriose + H2O 29% deacetylation Saccharomyces cerevisiae ?
-
 ?
3.5.1.41 N,N'-diacetylchitobiose + H2O 17% deacetylation Saccharomyces cerevisiae deacetylated chitooligosaccharides + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 monomer 1 * 43000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.5.1.41 Cda2p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 50
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.41 50
-
 24 h, 40% loss of activity Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 8
-
-
 Saccharomyces cerevisiae