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Literature summary extracted from

  • Salazar, O.; Molitor, J.; Lienqueo, M.E.; Asenjo, J.A.
    Overproduction, purification, and characterization of beta-1,3-glucanase type II in Escherichia coli (2001), Protein Expr. Purif., 23, 219-225.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39
-
 Cellulosimicrobium cellulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 0.688
-
 Laminarin pH 8.0, 37°C Cellulosimicrobium cellulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 43000
-
 x * 43000, recombinant protein with his6-tag, SDS-PAGE Cellulosimicrobium cellulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Cellulosimicrobium cellulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 recombinant enzyme with His6-tag, expression in Escherichia coli Cellulosimicrobium cellulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 8
-
 pH 8.0, 37°C Cellulosimicrobium cellulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Cellulosimicrobium cellulans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 43000, recombinant protein with his6-tag, SDS-PAGE Cellulosimicrobium cellulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.39 14.88
-
 Laminarin pH 8.0, 37°C Cellulosimicrobium cellulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.39 Cellulosimicrobium cellulans isoelectric focusing
-
 6