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Literature summary extracted from

  • Tull, D.; Gottschalk, T.E.; Svendsen, I.; Kramhoft, B.; Phillipson, B.A.; Bisgard-Frantzen, H.; Olsen, O.; Svensson, B.
    Extensive N-glycosylation reduces the thermal stability of a recombinant alkalophilic Bacillus alpha-amylase produced in Pichia pastoris (2001), Protein Expr. Purif., 21, 13-23.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Pichia pastoris Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 55331
-
 x * 55331, deduced from nucleotide sequence, native ABA Bacillus sp. (in: Bacteria)
3.2.1.1 57000
-
 x * 57000, SDS-PAGE, native ABA Bacillus sp. (in: Bacteria)
3.2.1.1 74000
-
 x * 74000, SDS-PAGE, recombinant ABA, due to glycosylation by Pichia pastoris Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Bacillus sp. (in: Bacteria)
-
 fragments of starch
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant ABA, diafiltration, Q-Sepharose Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 additional information
-
 4200 units/mg, one unit is defined as the amount of alpha-amylase that gives an increase of 0.001 in A620/ml for 15 min in a total volume of 5 ml Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 starch + H2O
-
 Bacillus sp. (in: Bacteria) fragments of starch
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 55331, deduced from nucleotide sequence, native ABA Bacillus sp. (in: Bacteria)
3.2.1.1 ? x * 57000, SDS-PAGE, native ABA Bacillus sp. (in: Bacteria)
3.2.1.1 ? x * 74000, SDS-PAGE, recombinant ABA, due to glycosylation by Pichia pastoris Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 ABA
-
 Bacillus sp. (in: Bacteria)
3.2.1.1 alkalophilic Bacillus alpha-amylase
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 68
-
 recombinant ABA, 50-60% loss of activity after 1 h, complete loss of activity after 100 min, native ABA, 20% loss of activity after 1 h, approx. 50% loss of activity after 100 min Bacillus sp. (in: Bacteria)
3.2.1.1 85
-
 complete loss of activity after 5 min Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6
-
 recombinant ABA, at 37°C Bacillus sp. (in: Bacteria)
3.2.1.1 7
-
 deglycosylated recombinant ABA, at 37°C Bacillus sp. (in: Bacteria)
3.2.1.1 8
-
 native ABA, at 37°C Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 5 10 native ABA at 37°C, approx. 30% of maximal activity at pH 5.0, approx. 90% of maximal activity at pH 10.0 Bacillus sp. (in: Bacteria)
3.2.1.1 5 10 recombinant ABA at 37°C, approx. 30% of maximal activity at pH 5.0, approx. 30% of maximal activity at pH 10.0 Bacillus sp. (in: Bacteria)
3.2.1.1 5 10 recombinant deglycosylated ABA at 37°C, approx. 50% of maximal activity at pH 5.0, approx. 30% of maximal activity at pH 10.0 Bacillus sp. (in: Bacteria)