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Literature summary extracted from

  • Suzuki, T.; Yasugi, M.; Arisaka, F.; Oshima, T.; Yamagishi, A.
    Cold-adaptation mechanism of mutant enzymes of 3-isopropylmalate dehydrogenase from Thermus thermophilus (2002), Protein Eng., 15, 471-476.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.85 V126A the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
1.1.1.85 V126E the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
1.1.1.85 V126F the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
1.1.1.85 V126G the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
1.1.1.85 V126I the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
1.1.1.85 V126L the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
1.1.1.85 V126M the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
1.1.1.85 V126S the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
1.1.1.85 V126T the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.002
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126G Thermus thermophilus
1.1.1.85 0.0026
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126A Thermus thermophilus
1.1.1.85 0.0027
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126T Thermus thermophilus
1.1.1.85 0.0032
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126S Thermus thermophilus
1.1.1.85 0.0044
-
 DL-3-isopropylmalate pH 8.0, 60°C, wild-type Thermus thermophilus
1.1.1.85 0.005
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126M Thermus thermophilus
1.1.1.85 0.0061
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126E Thermus thermophilus
1.1.1.85 0.0084
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126I Thermus thermophilus
1.1.1.85 0.0091
-
 NAD+ pH 8.0, 40°C, mutant V126S Thermus thermophilus
1.1.1.85 0.0092
-
 NAD+ pH 8.0, 40°C, mutant V126A Thermus thermophilus
1.1.1.85 0.0121
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126L Thermus thermophilus
1.1.1.85 0.0133
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126F Thermus thermophilus
1.1.1.85 0.022
-
 NAD+ pH 8.0, 40°C, mutant V126T Thermus thermophilus
1.1.1.85 0.024
-
 NAD+ pH 8.0, 40°C, mutant V126G Thermus thermophilus
1.1.1.85 0.025
-
 NAD+ pH 8.0, 40°C, wild-type Thermus thermophilus
1.1.1.85 0.0515
-
 NAD+ pH 8.0, 40°C, mutant V126E Thermus thermophilus
1.1.1.85 0.059
-
 NAD+ pH 8.0, 60°C, mutant V126A Thermus thermophilus
1.1.1.85 0.062
-
 NAD+ pH 8.0, 40°C, mutant V126I Thermus thermophilus
1.1.1.85 0.0725
-
 NAD+ pH 8.0, 60°C, mutant V126G Thermus thermophilus
1.1.1.85 0.095
-
 NAD+ pH 8.0, 60°C, mutant V126S Thermus thermophilus
1.1.1.85 0.103
-
 NAD+ pH 8.0, 60°C, mutant V126T Thermus thermophilus
1.1.1.85 0.109
-
 NAD+ pH 8.0, 60°C, mutant V126E Thermus thermophilus
1.1.1.85 0.111
-
 NAD+ pH 8.0, 40°C, mutant V126L Thermus thermophilus
1.1.1.85 0.144
-
 NAD+ pH 8.0, 40°C, mutant V126F Thermus thermophilus
1.1.1.85 0.166
-
 NAD+ pH 8.0, 60°C, wild-type Thermus thermophilus
1.1.1.85 0.198
-
 NAD+ pH 8.0, 60°C, mutant V126I Thermus thermophilus
1.1.1.85 0.378
-
 NAD+ pH 8.0, 60°C, mutant V126F Thermus thermophilus
1.1.1.85 0.391
-
 NAD+ pH 8.0, 60°C, mutant V126L Thermus thermophilus
1.1.1.85 1.01
-
 NAD+ pH 8.0, 40°C, mutant V126M Thermus thermophilus
1.1.1.85 1.97
-
 NAD+ pH 8.0, 60°C, mutant V126M Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 DL-3-isopropylmalate + NAD+
-
 Thermus thermophilus DL-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.85 3-isopropylmalate dehydrogenase
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 additional information
-
 additional information the cold adaption results from the destabilization of the ternary complex caused by increase in the volume of the residue at position 126 Thermus thermophilus
1.1.1.85 1.2
-
 NAD+ pH 8.0, 40°C, mutant V126A Thermus thermophilus
1.1.1.85 1.5
-
 NAD+ pH 8.0, 40°C, mutant V126S Thermus thermophilus
1.1.1.85 1.7
-
 NAD+ pH 8.0, 40°C, mutant V126G Thermus thermophilus
1.1.1.85 1.9
-
 NAD+ pH 8.0, 40°C, mutant V126T Thermus thermophilus
1.1.1.85 2.4
-
 NAD+ pH 8.0, 40°C, wild-type Thermus thermophilus
1.1.1.85 3.5
-
 NAD+ pH 8.0, 40°C, mutant V126E Thermus thermophilus
1.1.1.85 6
-
 NAD+ pH 8.0, 40°C, mutant V126I Thermus thermophilus
1.1.1.85 8.5
-
 NAD+ pH 8.0, 40°C, mutant V126L Thermus thermophilus
1.1.1.85 9
-
 NAD+ pH 8.0, 60°C, mutant V126A Thermus thermophilus
1.1.1.85 10
-
 NAD+ pH 8.0, 60°C, V126G Thermus thermophilus
1.1.1.85 11
-
 NAD+ pH 8.0, 40°C, mutant V126F Thermus thermophilus
1.1.1.85 13
-
 NAD+ pH 8.0, 60°C, mutant V126S Thermus thermophilus
1.1.1.85 15
-
 NAD+ pH 8.0, 60°C, mutant V126T Thermus thermophilus
1.1.1.85 18
-
 NAD+ pH 8.0, 40°C, mutant V126M Thermus thermophilus
1.1.1.85 20
-
 NAD+ pH 8.0, 60°C, wild-type Thermus thermophilus
1.1.1.85 21
-
 NAD+ pH 8.0, 60°C, mutant V126E Thermus thermophilus
1.1.1.85 30.5
-
 NAD+ pH 8.0, 60°C, mutant V126I Thermus thermophilus
1.1.1.85 42
-
 NAD+ pH 8.0, 60°C, mutant V126F Thermus thermophilus
1.1.1.85 44.5
-
 NAD+ pH 8.0, 60°C, mutant V126L Thermus thermophilus
1.1.1.85 54
-
 NAD+ pH 8.0, 60°C, mutant V126M Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Thermus thermophilus