BRENDA - Enzyme Database show

Cytidine deaminase from two extremophilic bacteria: cloning, expression and comparison of their structural stability

Cambi, A.; Vincenzetti, S.; De Sanctis, G.; Neuhard, J.; Natalini, P.; Vita, A.; Protein Eng. 14, 807-813 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.5
expression in Escherichia coli
Bacillus caldolyticus
3.5.4.5
expression in Escherichia coli
Sporosarcina psychrophila
General Stability
EC Number
General Stability
Organism
3.5.4.5
irreversible denaturation after 30 min at 72°C
Bacillus subtilis
3.5.4.5
irreversible denaturation at 72°C after 30 min
Sporosarcina psychrophila
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
14237
-
4 * 14237, calculation from nucleotide sequence
Bacillus caldolyticus
3.5.4.5
14600
-
4 * 14600, calculation from nucleotide sequence
Sporosarcina psychrophila
3.5.4.5
15000
-
4 * 15000, SDS-PAGE
Bacillus caldolyticus
3.5.4.5
15000
-
4 * 15000, SDS-PAGE
Sporosarcina psychrophila
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Bacillus caldolyticus
Q9R2S1
-
-
3.5.4.5
Bacillus subtilis
-
-
-
3.5.4.5
Sporosarcina psychrophila
Q9S3M0
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Bacillus caldolyticus
3.5.4.5
-
Sporosarcina psychrophila
Renatured (Commentary)
EC Number
Commentary
Organism
3.5.4.5
after partial denaturation at 72°C for 30 min and following renaturation on ice, the enzyme retains its enzymatic activity
Bacillus caldolyticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
deoxycytidine + H2O
-
657220
Bacillus subtilis
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
657220
Bacillus caldolyticus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
657220
Sporosarcina psychrophila
deoxyuridine + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
tetramer
4 * 14237, calculation from nucleotide sequence; 4 * 15000, SDS-PAGE
Bacillus caldolyticus
3.5.4.5
tetramer
4 * 14600, calculation from nucleotide sequence; 4 * 15000, SDS-PAGE
Sporosarcina psychrophila
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.5
33
-
-
Sporosarcina psychrophila
3.5.4.5
37
-
-
Bacillus subtilis
3.5.4.5
56
62
-
Bacillus caldolyticus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.4.5
6
70
6°C: 45% of maximal activity, 70°C: 35% of maximal activity
Sporosarcina psychrophila
3.5.4.5
12
66
12°C: about 55% of maximal activity, 66°C: about 65% of maximal activity
Bacillus subtilis
3.5.4.5
34
74
34°C: 45% of maximal activity, 74°C: 70% of maximal activity
Bacillus caldolyticus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.5
65
-
stable up to
Bacillus caldolyticus
3.5.4.5
72
-
30 min, irreversible denaturation
Bacillus subtilis
3.5.4.5
72
-
30 min, irreversible denaturation
Sporosarcina psychrophila
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.4.5
Bacillus subtilis
chromatofocusing
-
4.2
3.5.4.5
Sporosarcina psychrophila
chromatofocusing
-
4.2
3.5.4.5
Bacillus caldolyticus
chromatofocusing
-
4.9
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
expression in Escherichia coli
Bacillus caldolyticus
3.5.4.5
expression in Escherichia coli
Sporosarcina psychrophila
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.5
irreversible denaturation after 30 min at 72°C
Bacillus subtilis
3.5.4.5
irreversible denaturation at 72°C after 30 min
Sporosarcina psychrophila
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
14237
-
4 * 14237, calculation from nucleotide sequence
Bacillus caldolyticus
3.5.4.5
14600
-
4 * 14600, calculation from nucleotide sequence
Sporosarcina psychrophila
3.5.4.5
15000
-
4 * 15000, SDS-PAGE
Bacillus caldolyticus
3.5.4.5
15000
-
4 * 15000, SDS-PAGE
Sporosarcina psychrophila
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Bacillus caldolyticus
3.5.4.5
-
Sporosarcina psychrophila
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
after partial denaturation at 72°C for 30 min and following renaturation on ice, the enzyme retains its enzymatic activity
Bacillus caldolyticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
deoxycytidine + H2O
-
657220
Bacillus subtilis
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
657220
Bacillus caldolyticus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
657220
Sporosarcina psychrophila
deoxyuridine + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
tetramer
4 * 14237, calculation from nucleotide sequence; 4 * 15000, SDS-PAGE
Bacillus caldolyticus
3.5.4.5
tetramer
4 * 14600, calculation from nucleotide sequence; 4 * 15000, SDS-PAGE
Sporosarcina psychrophila
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.5
33
-
-
Sporosarcina psychrophila
3.5.4.5
37
-
-
Bacillus subtilis
3.5.4.5
56
62
-
Bacillus caldolyticus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.4.5
6
70
6°C: 45% of maximal activity, 70°C: 35% of maximal activity
Sporosarcina psychrophila
3.5.4.5
12
66
12°C: about 55% of maximal activity, 66°C: about 65% of maximal activity
Bacillus subtilis
3.5.4.5
34
74
34°C: 45% of maximal activity, 74°C: 70% of maximal activity
Bacillus caldolyticus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.5
65
-
stable up to
Bacillus caldolyticus
3.5.4.5
72
-
30 min, irreversible denaturation
Bacillus subtilis
3.5.4.5
72
-
30 min, irreversible denaturation
Sporosarcina psychrophila
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.4.5
Bacillus subtilis
chromatofocusing
-
4.2
3.5.4.5
Sporosarcina psychrophila
chromatofocusing
-
4.2
3.5.4.5
Bacillus caldolyticus
chromatofocusing
-
4.9