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Literature summary extracted from

  • Cambi, A.; Vincenzetti, S.; De Sanctis, G.; Neuhard, J.; Natalini, P.; Vita, A.
    Cytidine deaminase from two extremophilic bacteria: cloning, expression and comparison of their structural stability (2001), Protein Eng., 14, 807-813.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.5 expression in Escherichia coli [Bacillus] caldolyticus
3.5.4.5 expression in Escherichia coli Sporosarcina psychrophila

General Stability

EC Number General Stability Organism
3.5.4.5 irreversible denaturation after 30 min at 72°C Bacillus subtilis
3.5.4.5 irreversible denaturation at 72°C after 30 min Sporosarcina psychrophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 14237
-
 4 * 14237, calculation from nucleotide sequence [Bacillus] caldolyticus
3.5.4.5 14600
-
 4 * 14600, calculation from nucleotide sequence Sporosarcina psychrophila
3.5.4.5 15000
-
 4 * 15000, SDS-PAGE [Bacillus] caldolyticus
3.5.4.5 15000
-
 4 * 15000, SDS-PAGE Sporosarcina psychrophila

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Bacillus subtilis
-
-
-
3.5.4.5 Sporosarcina psychrophila Q9S3M0
-
-
3.5.4.5 [Bacillus] caldolyticus Q9R2S1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5
-
 [Bacillus] caldolyticus
3.5.4.5
-
 Sporosarcina psychrophila

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.4.5 after partial denaturation at 72°C for 30 min and following renaturation on ice, the enzyme retains its enzymatic activity [Bacillus] caldolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 deoxycytidine + H2O
-
 Bacillus subtilis deoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 [Bacillus] caldolyticus deoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Sporosarcina psychrophila deoxyuridine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 tetramer 4 * 15000, SDS-PAGE [Bacillus] caldolyticus
3.5.4.5 tetramer 4 * 15000, SDS-PAGE Sporosarcina psychrophila
3.5.4.5 tetramer 4 * 14237, calculation from nucleotide sequence [Bacillus] caldolyticus
3.5.4.5 tetramer 4 * 14600, calculation from nucleotide sequence Sporosarcina psychrophila

Synonyms

EC Number Synonyms Comment Organism
3.5.4.5 CDABcald
-
 [Bacillus] caldolyticus
3.5.4.5 CDABpsy
-
 Sporosarcina psychrophila
3.5.4.5 CDABsub
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.5 33
-
-
 Sporosarcina psychrophila
3.5.4.5 37
-
-
 Bacillus subtilis
3.5.4.5 56 62
-
 [Bacillus] caldolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.4.5 6 70 6°C: 45% of maximal activity, 70°C: 35% of maximal activity Sporosarcina psychrophila
3.5.4.5 12 66 12°C: about 55% of maximal activity, 66°C: about 65% of maximal activity Bacillus subtilis
3.5.4.5 34 74 34°C: 45% of maximal activity, 74°C: 70% of maximal activity [Bacillus] caldolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.5 65
-
 stable up to [Bacillus] caldolyticus
3.5.4.5 72
-
 30 min, irreversible denaturation Bacillus subtilis
3.5.4.5 72
-
 30 min, irreversible denaturation Sporosarcina psychrophila

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.4.5 Bacillus subtilis chromatofocusing
-
 4.2
3.5.4.5 Sporosarcina psychrophila chromatofocusing
-
 4.2
3.5.4.5 [Bacillus] caldolyticus chromatofocusing
-
 4.9