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Literature summary extracted from

  • Stewart, R.J.; Varghese, J.N.; Garrett, T.P.J.; Hoj, P.B.; Fincher, G.B.
    Mutant barley (1-3,1-4)-beta-glucan endohydrolases with enhanced thermostability (2001), Protein Eng., 14, 245-253.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 expression in Escherichia coli Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.73 A79P decrease in thermal stability Hordeum vulgare
3.2.1.73 F85Y decrease in thermal stability Hordeum vulgare
3.2.1.73 G44R decrease in thermal stability Hordeum vulgare
3.2.1.73 H300P increase in thermal stability Hordeum vulgare
3.2.1.73 K23R decrease in thermal stability Hordeum vulgare
3.2.1.73 M298K thermal stabiltiy similar to wild type Hordeum vulgare
3.2.1.73 N290H slight increase in thermal stability Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 33500
-
 x * 33500, SDS-PAGE, recombinant enzyme with His-tag Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Hordeum vulgare
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 14.5
-
 37°C, pH 5.0 Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
3.2.1.73 4°C, 50 mM sodium phosphate, pH 7.8, 300 mM sodium chloride, stable for several weeks Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 (1-3,1-4)-beta-D-glucan + H2O i.e. barley (1-3,1-4)-beta-glucan Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x * 33500, SDS-PAGE, recombinant enzyme with His-tag Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 44.5
-
 15 min, 50% of activity, mutant G44R Hordeum vulgare
3.2.1.73 46
-
 15 min, 50% of activity, mutant K23R Hordeum vulgare
3.2.1.73 46.1
-
 15 min, 50% of activity, mutant A79P Hordeum vulgare
3.2.1.73 46.2
-
 15 min, 50% of activity, mutant F85Y Hordeum vulgare
3.2.1.73 47.5
-
 15 min, 50% of activity, wild type Hordeum vulgare
3.2.1.73 47.9
-
 15 min, 50% of activity, mutant M298K, mutant T17D Hordeum vulgare
3.2.1.73 48.2
-
 15 min, 50% of activity, mutant N290H Hordeum vulgare
3.2.1.73 51.2
-
 15 min, 50% of activity, mutant H300P Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 4.5
-
 wild type, mutant H300P Hordeum vulgare