EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.73 | expression in Escherichia coli | Hordeum vulgare |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.73 | A79P | decrease in thermal stability | Hordeum vulgare |
3.2.1.73 | F85Y | decrease in thermal stability | Hordeum vulgare |
3.2.1.73 | G44R | decrease in thermal stability | Hordeum vulgare |
3.2.1.73 | H300P | increase in thermal stability | Hordeum vulgare |
3.2.1.73 | K23R | decrease in thermal stability | Hordeum vulgare |
3.2.1.73 | M298K | thermal stabiltiy similar to wild type | Hordeum vulgare |
3.2.1.73 | N290H | slight increase in thermal stability | Hordeum vulgare |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 33500 | - |
x * 33500, SDS-PAGE, recombinant enzyme with His-tag | Hordeum vulgare |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.73 | Hordeum vulgare | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 14.5 | - |
37°C, pH 5.0 | Hordeum vulgare |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.73 | 4°C, 50 mM sodium phosphate, pH 7.8, 300 mM sodium chloride, stable for several weeks | Hordeum vulgare |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.73 | (1-3,1-4)-beta-D-glucan + H2O | i.e. barley (1-3,1-4)-beta-glucan | Hordeum vulgare | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.73 | ? | x * 33500, SDS-PAGE, recombinant enzyme with His-tag | Hordeum vulgare |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 44.5 | - |
15 min, 50% of activity, mutant G44R | Hordeum vulgare |
3.2.1.73 | 46 | - |
15 min, 50% of activity, mutant K23R | Hordeum vulgare |
3.2.1.73 | 46.1 | - |
15 min, 50% of activity, mutant A79P | Hordeum vulgare |
3.2.1.73 | 46.2 | - |
15 min, 50% of activity, mutant F85Y | Hordeum vulgare |
3.2.1.73 | 47.5 | - |
15 min, 50% of activity, wild type | Hordeum vulgare |
3.2.1.73 | 47.9 | - |
15 min, 50% of activity, mutant M298K, mutant T17D | Hordeum vulgare |
3.2.1.73 | 48.2 | - |
15 min, 50% of activity, mutant N290H | Hordeum vulgare |
3.2.1.73 | 51.2 | - |
15 min, 50% of activity, mutant H300P | Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 4.5 | - |
wild type, mutant H300P | Hordeum vulgare |