BRENDA - Enzyme Database

Ecto-diadenosine polyphosphate hydrolase activity on human prostasomes

Minelli, A.; Allegrucci, C.; Liguori, L.; Ronquist, G.; Prostate 51, 1-9 (2002)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
Ap5
-
Homo sapiens
3.6.1.17
diadenosine 5',5'''-P1,P4-tetraphosphate
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.038
-
Ap4A
pH 7.4, 37C
Homo sapiens
3.6.1.17
0.04
-
Ap6A
pH 7.4, 37C
Homo sapiens
3.6.1.17
0.098
-
Ap5A
pH 7.4, 37C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.17
membrane
-
Homo sapiens
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Ca2+
activated by physiological concentration, maximal activity at 2 mM
Homo sapiens
3.6.1.17
Mg2+
activated by physiological concentration, maximal activity at 2 mM
Homo sapiens
3.6.1.17
Mn2+
activated by physiological concentration, maximal activity at 0.1 mM
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.17
additional information
spermatozoa do not possess this hydrolytic activity, but they can aquire it after fusion with prostasomes
Homo sapiens
-
3.6.1.17
prostasome
bound to prostasome-membranes through a GPI-anchor
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
Ap6A + H2O
-
657213
Homo sapiens
Ap5 + AMP
-
-
-
?
3.6.1.17
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
657213
Homo sapiens
ATP + AMP
-
-
-
?
3.6.1.17
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
657213
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
7
8
degradation of Ap4A
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.17
6.1
8.6
pH 6.1: about 75% of maximal activity, pH 8.6: about 10% of maximal activity, degradation of Ap4A
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
Ap5
-
Homo sapiens
3.6.1.17
diadenosine 5',5'''-P1,P4-tetraphosphate
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.038
-
Ap4A
pH 7.4, 37C
Homo sapiens
3.6.1.17
0.04
-
Ap6A
pH 7.4, 37C
Homo sapiens
3.6.1.17
0.098
-
Ap5A
pH 7.4, 37C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.17
membrane
-
Homo sapiens
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Ca2+
activated by physiological concentration, maximal activity at 2 mM
Homo sapiens
3.6.1.17
Mg2+
activated by physiological concentration, maximal activity at 2 mM
Homo sapiens
3.6.1.17
Mn2+
activated by physiological concentration, maximal activity at 0.1 mM
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.17
additional information
spermatozoa do not possess this hydrolytic activity, but they can aquire it after fusion with prostasomes
Homo sapiens
-
3.6.1.17
prostasome
bound to prostasome-membranes through a GPI-anchor
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
Ap6A + H2O
-
657213
Homo sapiens
Ap5 + AMP
-
-
-
?
3.6.1.17
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
657213
Homo sapiens
ATP + AMP
-
-
-
?
3.6.1.17
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
657213
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
7
8
degradation of Ap4A
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.17
6.1
8.6
pH 6.1: about 75% of maximal activity, pH 8.6: about 10% of maximal activity, degradation of Ap4A
Homo sapiens