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Literature summary extracted from

  • Cho, Y.J.; Yun, J.W.
    Purification and characterization of an endoinulinase from Xanthomonas oryzae No.5 (2002), Process Biochem., 37, 1325-1331.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.7 nutrition production of oligosaccharides, which are soluble dietary fibers and/or a functional sweeteners Xanthomonas oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.7 inulin concentrations above 125 g/l Xanthomonas oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.7 additional information
-
 additional information inulin, 16.7 g/l, pH 7.5, at 50ºC Xanthomonas oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.7 extracellular
-
 Xanthomonas oryzae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.7 139000
-
 SDS-PAGE Xanthomonas oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.7 inulin + H2O Xanthomonas oryzae
-
 inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O Xanthomonas oryzae No.5
-
 inulooligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Xanthomonas oryzae
-
 No.5
-
3.2.1.7 Xanthomonas oryzae No.5
-
 No.5
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.7 no modification
-
 Xanthomonas oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.7 by ammonium sulfate precipitation, DEAE-Sephacel column and phenyl-Sepharose column, 28.7fold Xanthomonas oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.7 culture medium
-
 Xanthomonas oryzae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.7 1407
-
 pH 6.0, at 45ºC Xanthomonas oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O
-
 Xanthomonas oryzae inulooligosaccharides
-
 ?
3.2.1.7 inulin + H2O
-
 Xanthomonas oryzae No.5 inulooligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.7 endoinulinase
-
 Xanthomonas oryzae
3.2.1.7 fructofuranosyl hydrolase
-
 Xanthomonas oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 50
-
 pH 6.0 Xanthomonas oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.7 45
-
 pH 6.0, up to 45ºC Xanthomonas oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 7.5
-
 at 45ºC Xanthomonas oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.7 6 9 at 20ºC Xanthomonas oryzae