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Literature summary extracted from

  • Saha, B.C.
    Production, purification and properties of xylanase from a newly isolated Fusarium proliferatum (2002), Process Biochem., 37, 1279-1284.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+
-
 Fusarium proliferatum
3.2.1.8 DTT
-
 Fusarium proliferatum
3.2.1.8 Mn2+
-
 Fusarium proliferatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Fusarium proliferatum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 additional information no metal requirement for activity and stability Fusarium proliferatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 22400
-
 1 * 22400, SDS-PAGE Fusarium proliferatum
3.2.1.8 25000
-
 gel filtration Fusarium proliferatum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Fusarium proliferatum
-
-
-
3.2.1.8 Fusarium proliferatum NRRL 26517
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 975fold from the culture supernatant, to homogeneity by ammonium sulfate precipitation and several chromatographic steps Fusarium proliferatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 591
-
 purified enzyme Fusarium proliferatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 xylan + H2O diverse xylan substrates, best substrate is wheat arabinoxylan, overview Fusarium proliferatum xylobiose + ? no formation of xylose ?
3.2.1.8 xylan + H2O diverse xylan substrates, best substrate is wheat arabinoxylan, overview Fusarium proliferatum NRRL 26517 xylobiose + ? no formation of xylose ?
3.2.1.8 xylan + H2O xylan substrate from oat spelt Fusarium proliferatum xylobiose + xylotetraose + ? no formation of xylose ?
3.2.1.8 xylan + H2O xylan substrate from oat spelt Fusarium proliferatum NRRL 26517 xylobiose + xylotetraose + ? no formation of xylose ?
3.2.1.8 xylan + H2O xylan substrate from birchwood Fusarium proliferatum xylobiose + xylotriose + ? no formation of xylose ?
3.2.1.8 xylan + H2O xylan substrate from birchwood Fusarium proliferatum NRRL 26517 xylobiose + xylotriose + ? no formation of xylose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 22400, SDS-PAGE Fusarium proliferatum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Fusarium proliferatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 55
-
-
 Fusarium proliferatum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 40 70 89% of maximal activity at 60°C and 14% at 70°C Fusarium proliferatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 55
-
 fully stable up to, 30 min, pH 5.0 Fusarium proliferatum
3.2.1.8 60
-
 loss of 78% activity after 30 min at pH 5.0 Fusarium proliferatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5 5.5
-
 Fusarium proliferatum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 5 7.5 fully stable, 1 h at 40°C, and 30 min at 55°C Fusarium proliferatum