EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.8.1.7 | W137F | structure and reaction simulations compared to the wild-type enzyme | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.7 | 4-chlorobenzoyl-CoA + H2O | Pseudomonas sp. | - |
4-hydroxybenzoyl-CoA + chloride | - |
? | |
3.8.1.7 | 4-chlorobenzoyl-CoA + H2O | Pseudomonas sp. CBS-3 | - |
4-hydroxybenzoyl-CoA + chloride | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.8.1.7 | Pseudomonas sp. | - |
- |
- |
3.8.1.7 | Pseudomonas sp. CBS-3 | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.8.1.7 | 4-chlorobenzoyl-CoA + H2O = 4-hydroxybenzoyl CoA + chloride | specific for dehalogenation at the 4-position, can dehalogenate substrates bearing fluorine, chlorine, bromine and iodine in the 4-position, SNAr reaction mechanism, the substrate is bound in the active site primariliy formed by 2 of 3 subunits, reaction is initiated by the nucleophilic attack at the C4 of the benzoyl group of the substrate by carboxylate of Asp145 to form a Meisenheimer intermediate, general base for the hydrolysis is His90, the benzoyl group is surrounded by F64, F82, W89, and W137, active site conformations of wild-type and mutant W137F | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.7 | 4-chlorobenzoyl-CoA + H2O | - |
Pseudomonas sp. | 4-hydroxybenzoyl-CoA + chloride | - |
? | |
3.8.1.7 | 4-chlorobenzoyl-CoA + H2O | - |
Pseudomonas sp. CBS-3 | 4-hydroxybenzoyl-CoA + chloride | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.8.1.7 | 4-CBA-CoA dehalogenase | - |
Pseudomonas sp. |