BRENDA - Enzyme Database

Expression of catalytically active barley glutamyl tRNAGlu reductase in Escherichia coli as a fusion protein with glutathione S-transferase

Vothknecht, U.C.; Kannangara, C.G.; von Wettstein, D.; Proc. Natl. Acad. Sci. USA 93, 9287-9291 (1996)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.70
expression in Escherichia coli as a fusion protein with glutathione S-transferase
Hordeum vulgare
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
hemin
50% inhibition at 0.0015 mM
Hordeum vulgare
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
60000
-
4 * 60000, SDS-PAGE
Hordeum vulgare
1.2.1.70
250000
-
gel filtration
Hordeum vulgare
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Hordeum vulgare
the enzyme catalyzes the initial step of tetrapyrrole biosynthesis
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Hordeum vulgare
Q42843
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.70
fusion protein with glutathione S-transferase
Hordeum vulgare
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.70
0.12
-
fusion protein with glutathione S-transferase
Hordeum vulgare
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the enzyme catalyzes the initial step of tetrapyrrole biosynthesis
657174
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the fusion protein with glutathione S-transferase uses tRNAGlu from Hordeum vulgare chloroplast preferentially to Escherichia coli tRNAGlu
657174
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.70
tetramer
4 * 60000, SDS-PAGE
Hordeum vulgare
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
heme
the fusion protein with glutathione S-transferase contains heme, which can be reduced by NADPH and oxidized by air
Hordeum vulgare
1.2.1.70
NADPH
-
Hordeum vulgare
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
expression in Escherichia coli as a fusion protein with glutathione S-transferase
Hordeum vulgare
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
heme
the fusion protein with glutathione S-transferase contains heme, which can be reduced by NADPH and oxidized by air
Hordeum vulgare
1.2.1.70
NADPH
-
Hordeum vulgare
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
hemin
50% inhibition at 0.0015 mM
Hordeum vulgare
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
60000
-
4 * 60000, SDS-PAGE
Hordeum vulgare
1.2.1.70
250000
-
gel filtration
Hordeum vulgare
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Hordeum vulgare
the enzyme catalyzes the initial step of tetrapyrrole biosynthesis
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
fusion protein with glutathione S-transferase
Hordeum vulgare
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.70
0.12
-
fusion protein with glutathione S-transferase
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the enzyme catalyzes the initial step of tetrapyrrole biosynthesis
657174
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the fusion protein with glutathione S-transferase uses tRNAGlu from Hordeum vulgare chloroplast preferentially to Escherichia coli tRNAGlu
657174
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.70
tetramer
4 * 60000, SDS-PAGE
Hordeum vulgare