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Literature summary extracted from

  • Mustafi, D.; Bekesi, A.; Vertessy, B.G.; Makinen, M.W.
    Catalytic and structural role of the metal ion in dUTP pyrophosphatase (2003), Proc. Natl. Acad. Sci. USA, 100, 5670-5675.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.23 bis(acetylacetonato)oxovanadium(IV)
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required Escherichia coli
3.6.1.23 Mn2+ can replace for Mg2+ Escherichia coli
3.6.1.23 VO2+ results a higher activity than Mg2+ or Mn2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Escherichia coli removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Escherichia coli dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA Escherichia coli dUMP + diphosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 4.5
-
 dUTP pH 7.5, 25°C, in the absence of added metal ions Escherichia coli
3.6.1.23 14.7
-
 dUTP pH 7.5, 25°C, in the presence of Mg2+ or Mn2+ Escherichia coli
3.6.1.23 24.2
-
 dUTP pH 7.5, 25°C, in the presence of bis(acetylacetonato)oxovanadium(IV) Escherichia coli
3.6.1.23 27.3
-
 dUTP pH 7.5, 25°C, in the presence of VO2+ or bis(acetylacetonato)oxovanadium(IV) Escherichia coli