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Literature summary extracted from

  • Dong, J.L.; Zhang, Y.Z.
    Purification and characterization of two laccase isoenzymes from a ligninolytic fungus Trametes gallica (2004), Prep. Biochem. Biotechnol., 34, 179-194.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 azide complete inhibition Phlebia fascicularia
1.10.3.2 cyanide complete inhibition Phlebia fascicularia
1.10.3.2 EDTA partiall inhibition Phlebia fascicularia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.00086
-
 2,6-dimethoxyphenol Lac II, 70°C, pH 3.0 Phlebia fascicularia
1.10.3.2 0.00118
-
 2,6-dimethoxyphenol Lac I, 70°C, pH 3.0 Phlebia fascicularia
1.10.3.2 0.4
-
 guaiacol Lac II, 70°C, pH 4.0 Phlebia fascicularia
1.10.3.2 0.405
-
 guaiacol Lac I, 70°C, pH 4.0 Phlebia fascicularia
1.10.3.2 0.41
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Lac II, 70°C, pH 2.2 Phlebia fascicularia
1.10.3.2 0.42
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Lac I, 70°C, pH 2.2 Phlebia fascicularia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper contains 4 copper atoms per protein molecule Phlebia fascicularia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 60000
-
 gel filtration, isozymes Lac I and Lac II Phlebia fascicularia

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Phlebia fascicularia
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein Lac I, 3.6%, Lac II 4% carbohydrate content Phlebia fascicularia

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 isozymes Lac I, Lac II Phlebia fascicularia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture supernatant two isozymes, growth on tryptone and glucose Phlebia fascicularia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Phlebia fascicularia ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Phlebia fascicularia ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Phlebia fascicularia ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 60000, SDS-PAGE, isozymes Lac I and Lac II Phlebia fascicularia

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 Lac I
-
 no activity in Cavia porcellus
1.10.3.2 Lac II
-
 no activity in Cavia porcellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 70
-
 Lac I and Lac II Phlebia fascicularia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 stable below Phlebia fascicularia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.2
-
 Lac I and Lac II, substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Phlebia fascicularia
1.10.3.2 3
-
 Lac I and Lac II, substrate 2,6-dimethoxyphenol Phlebia fascicularia
1.10.3.2 4
-
 Lac I and Lac II, substrate guaiacol Phlebia fascicularia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 6 9 90% residual activity after 24 h incungation Phlebia fascicularia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Phlebia fascicularia isoelectric focusing, isozyme Lac II
-
 3
1.10.3.2 Phlebia fascicularia isoelectric focusing, isozyme Lac I 4.8 4.7