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Literature summary extracted from

  • Mo, B.; Bewley, J.D.
    beta-Mannosidase (EC 3.2.1.25) activity during and following germination of tomato (Lycopersicon esculentum Mill.) seeds. Purification, cloning and characterization (2002), Planta, 215, 141-152.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.25
-
 Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 0.55
-
 p-nitrophenyl beta-D-mannopyranoside
-
 Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.25 cell wall ionically associated with a matrix fraction, most likely the cell wall Solanum lycopersicum 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 56000
-
 x * 56000, SDS-PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.25 additional information Solanum lycopersicum coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan ?
-
 ?
3.2.1.25 additional information Solanum lycopersicum Mill. coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Solanum lycopersicum
-
 Mill.
-
3.2.1.25 Solanum lycopersicum Mill.
-
 Mill.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
 Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.25 seed increase in activity following germination Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.25 0.307
-
-
 Solanum lycopersicum

Storage Stability

EC Number Storage Stability Organism
3.2.1.25 -80°C, pH 5.0, chromatofocusing elution buffer, loss of activity within 1 month Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 additional information coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan Solanum lycopersicum ?
-
 ?
3.2.1.25 additional information coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan Solanum lycopersicum Mill. ?
-
 ?
3.2.1.25 p-nitrophenyl beta-D-mannopyranoside + H2O
-
 Solanum lycopersicum p-nitrophenol + beta-D-mannopyranose
-
 ?
3.2.1.25 p-nitrophenyl beta-D-mannopyranoside + H2O
-
 Solanum lycopersicum Mill. p-nitrophenol + beta-D-mannopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.25 ? x * 56000, SDS-PAGE Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.25 40
-
-
 Solanum lycopersicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.25 40 55 40°C: optimum, at optimal pH active up to 55°C Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 5
-
-
 Solanum lycopersicum