EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.30.2 | Mg2+ | - |
Hordeum vulgare |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.2 | dsDNA + H2O | Hordeum vulgare | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.30.2 | Hordeum vulgare | - |
barley | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.30.2 | affinity chromatography, 63fold purification | Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.30.2 | microspore | at the mid-to-late uninucleate stage | Hordeum vulgare | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.2 | dsDNA + H2O | - |
Hordeum vulgare | ? | - |
? | |
3.1.30.2 | additional information | poly(dC)poly(dG) is not substrate for the enzyme | Hordeum vulgare | ? | - |
? | |
3.1.30.2 | poly(A) + H2O | more rapidly degraded than native DNA | Hordeum vulgare | ? | - |
? | |
3.1.30.2 | poly(C) + H2O | more rapidly degraded than native DNA | Hordeum vulgare | ? | - |
? | |
3.1.30.2 | poly(dA) + H2O | more rapidly degraded than native DNA | Hordeum vulgare | ? | - |
? | |
3.1.30.2 | poly(dA)poly(dT) + H2O | more rapidly degraded than native DNA | Hordeum vulgare | ? | - |
? | |
3.1.30.2 | poly(dC) + H2O | - |
Hordeum vulgare | ? | - |
? | |
3.1.30.2 | poly(dT) + H2O | - |
Hordeum vulgare | ? | - |
? | |
3.1.30.2 | poly(G) + H2O | - |
Hordeum vulgare | ? | - |
? | |
3.1.30.2 | poly(I) + H2O | - |
Hordeum vulgare | ? | - |
? | |
3.1.30.2 | RNA + H2O | - |
Hordeum vulgare | ? | - |
? | |
3.1.30.2 | ssDNA + H2O | more rapidly degraded than native DNA | Hordeum vulgare | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.30.2 | BMN | barley microspore nuclease | Hordeum vulgare |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | 70 | - |
- |
Hordeum vulgare |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | 30 | 70 | activity increases exponentially | Hordeum vulgare |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | - |
60 | pre-incubation of the enzyme for 30 min, stable | Hordeum vulgare |
3.1.30.2 | 65 | - |
pre-incubation of the enzyme for 30 min, 60% of the activity | Hordeum vulgare |
3.1.30.2 | 80 | - |
above, substantial inactivation | Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | 5.6 | - |
- |
Hordeum vulgare |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | 4 | 8 | substantially reduced activity above and below | Hordeum vulgare |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | additional information | - |
basic pH favors enzymatic stability | Hordeum vulgare |
3.1.30.2 | 8 | - |
above, residual activity greater than 50% | Hordeum vulgare |