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Literature summary extracted from

  • Marchetti, S.; Zaina, G.; Chiaba, C.; Pappalardo, C.; Pitotti, A.
    Isolation and characterization of an endonuclease synthesized by barley (Hordeum vulgare L.) uninucleate microspores (2001), Planta, 213, 199-206.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.2 Mg2+
-
Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.2 dsDNA + H2O Hordeum vulgare
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.2 Hordeum vulgare
-
barley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.2 affinity chromatography, 63fold purification Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.30.2 microspore at the mid-to-late uninucleate stage Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.2 dsDNA + H2O
-
Hordeum vulgare ?
-
?
3.1.30.2 additional information poly(dC)poly(dG) is not substrate for the enzyme Hordeum vulgare ?
-
?
3.1.30.2 poly(A) + H2O more rapidly degraded than native DNA Hordeum vulgare ?
-
?
3.1.30.2 poly(C) + H2O more rapidly degraded than native DNA Hordeum vulgare ?
-
?
3.1.30.2 poly(dA) + H2O more rapidly degraded than native DNA Hordeum vulgare ?
-
?
3.1.30.2 poly(dA)poly(dT) + H2O more rapidly degraded than native DNA Hordeum vulgare ?
-
?
3.1.30.2 poly(dC) + H2O
-
Hordeum vulgare ?
-
?
3.1.30.2 poly(dT) + H2O
-
Hordeum vulgare ?
-
?
3.1.30.2 poly(G) + H2O
-
Hordeum vulgare ?
-
?
3.1.30.2 poly(I) + H2O
-
Hordeum vulgare ?
-
?
3.1.30.2 RNA + H2O
-
Hordeum vulgare ?
-
?
3.1.30.2 ssDNA + H2O more rapidly degraded than native DNA Hordeum vulgare ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.2 BMN barley microspore nuclease Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.2 70
-
-
Hordeum vulgare

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.30.2 30 70 activity increases exponentially Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.30.2
-
60 pre-incubation of the enzyme for 30 min, stable Hordeum vulgare
3.1.30.2 65
-
pre-incubation of the enzyme for 30 min, 60% of the activity Hordeum vulgare
3.1.30.2 80
-
above, substantial inactivation Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.2 5.6
-
-
Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.30.2 4 8 substantially reduced activity above and below Hordeum vulgare

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.30.2 additional information
-
basic pH favors enzymatic stability Hordeum vulgare
3.1.30.2 8
-
above, residual activity greater than 50% Hordeum vulgare