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Literature summary extracted from
- Purification and characterization of a hydroxamic acid glucoside beta-glucosidase from wheat (Triticum aestivum L.) seedlings (2000), Planta, 210, 432-438.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.24 | - |
esculin | 30°C, pH 5.5 | Triticum aestivum |  |
| 3.2.1.21 | 0.27 | - |
2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one-beta-D-glucoside | 30°C, pH 5.5 | Triticum aestivum | |
| 3.2.1.21 | 0.32 | - |
2-hydroxy-7-methoxy-1,4-benzoxazin-3-one-beta-D-glucoside | 30°C, pH 5.5 | Triticum aestivum | |
| 3.2.1.21 | 0.67 | - |
p-nitrophenyl-beta-D-fucopyranoside | 30°C, pH 5.5 | Triticum aestivum | |
| 3.2.1.21 | 1.34 | - |
2,4-dihydroxy-1,4-benzoxazin-3-one glucoside | 30°C, pH 5.5 | Triticum aestivum | |
| 3.2.1.21 | 1.7 | - |
p-nitrophenyl-beta-D-glucopyranoside | 30°C, pH 5.5 | Triticum aestivum | |
| 3.2.1.21 | 1.78 | - |
p-nitrophenyl-beta-D-glucopyranoside | 30°C, pH 5.5 | Triticum aestivum | |
| 3.2.1.21 | 2 | - |
2-hydroxy-1,4-benzoxazin-3-one-beta-D-glucoside | 30°C, pH 5.5 | Triticum aestivum | |
| 3.2.1.21 | 3.1 | - |
p-nitrophenyl-beta-D-xylopyranoside | 30°C, pH 5.5 | Triticum aestivum | |
| 3.2.1.182 | 0.24 | - |
esculin | pH 5.5, 30°C | Triticum aestivum | |
| 3.2.1.182 | 0.27 | - |
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside | pH 5.5, 30°C | Triticum aestivum | |
| 3.2.1.182 | 0.67 | - |
4-nitrophenyl beta-D-fucoside | pH 5.5, 30°C | Triticum aestivum | |
| 3.2.1.182 | 1.34 | - |
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside | pH 5.5, 30°C | Triticum aestivum | |
| 3.2.1.182 | 1.7 | - |
4-nitrophenyl beta-D-glucoside | pH 5.5, 30°C | Triticum aestivum | |
| 3.2.1.182 | 1.78 | - |
4-nitrophenyl beta-D-galactoside | pH 5.5, 30°C | Triticum aestivum | |
| 3.2.1.182 | 2.02 | - |
7-methoxy-3-oxo-3,4-dihydro-2H-1,4 benzoxazin-2-yl beta-D-glucopyranoside | pH 5.5, 30°C | Triticum aestivum | |
| 3.2.1.182 | 3.11 | - |
4-nitrophenyl beta-D-xyloside | pH 5.5, 30°C | Triticum aestivum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 260000 | 300000 | gel filtration | Triticum aestivum |
| 3.2.1.182 | 60000 | - |
enzyme is found to be present as oligomeric forms with a molecular mass of 260-300 kDa comprising 60- and 58-kDa monomers | Triticum aestivum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Triticum aestivum | - |
- |
- |
| 3.2.1.182 | Triticum aestivum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | cryoprecipitation, Accell Plus CM, Superdex 200 | Triticum aestivum |
| 3.2.1.182 | using gel filtration | Triticum aestivum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | seedling | - |
Triticum aestivum | - |
| 3.2.1.182 | root | - |
Triticum aestivum | - |
| 3.2.1.182 | seedling | isolated from | Triticum aestivum | - |
| 3.2.1.182 | shoot | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40.3 | - |
hydrolysis of 2,4-dihydroxy-1,4-benzoxazin-3-one-beta-D-glucoside | Triticum aestivum |
| 3.2.1.21 | 202.3 | - |
hydrolysis of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one-beta-D-glucoside | Triticum aestivum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 2,4-dihydroxy-1,4-benzoxazin-3-one-beta-D-glucoside + H2O | - |
Triticum aestivum | 2,4-dihydroxy-1,4-benzoxazin-3-one + beta-D-glucose | - |
? | |
| 3.2.1.21 | 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside + H2O | - |
Triticum aestivum | 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one + beta-D-glucose | - |
? | |
| 3.2.1.21 | 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one-beta-D-glucoside + H2O | - |
Triticum aestivum | 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one + beta-D-glucose | - |
? | |
| 3.2.1.21 | 2-hydroxy-1,4-benzoxazin-3-one-beta-D-glucoside + H2O | - |
Triticum aestivum | 2-hydroxy-1,4-benzoxazin-3-one + beta-D-glucose | - |
? | |
| 3.2.1.21 | 2-hydroxy-7-methoxy-1,4-benzoxazin-3-one-beta-D-glucoside + H2O | - |
Triticum aestivum | 2-hydroxy-7-methoxy-1,4-benzoxazin-3-one + beta-D-glucose | - |
? | |
| 3.2.1.21 | 2-O-beta-D-glucopyranosyl-4-hydroxy-7-demethoxy-1,4-benzoxazin-3-one + H2O | - |
Triticum aestivum | 2,4-dihydroxy-7-demethoxy-1,4-benzoxazin-3-one + beta-D-glucose | - |
? | |
| 3.2.1.21 | apigenin-beta-D-glucoside + H2O | - |
Triticum aestivum | apigenin + beta-D-glucose | - |
? | |
| 3.2.1.21 | biochanin A 7-O-beta-D-glucopyranoside + H2O | - |
Triticum aestivum | biochanin A + beta-D-glucose | - |
? | |
| 3.2.1.21 | esculin + H2O | - |
Triticum aestivum | beta-D-glucose + esculetin | - |
? | |
| 3.2.1.21 | genistein-beta-D-glucoside + H2O | - |
Triticum aestivum | genistein + beta-D-glucose | - |
? | |
| 3.2.1.21 | luteolin-beta-D-glucoside + H2O | weak activity | Triticum aestivum | luteolin + beta-D-glucose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-beta-D-fucopyranoside + H2O | weak activity | Triticum aestivum | p-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-beta-D-galactopyranoside + H2O | weak activity | Triticum aestivum | p-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Triticum aestivum | p-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-beta-D-xylopyranoside + H2O | weak activity | Triticum aestivum | p-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.182 | 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside + H2O | the maximum activity with DIMBOA-Glc is 10times higher in shoots and 7times higher in roots than that with DIBOA-Glc | Triticum aestivum | 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose | - |
? | |
| 3.2.1.182 | 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside + H2O | the maximum activity with DIMBOA-Glc is 10times higher in shoots and 7times higher in roots than that with DIBOA-Glc | Triticum aestivum | 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose | - |
? | |
| 3.2.1.182 | 4-nitrophenyl beta-D-fucoside + H2O | - |
Triticum aestivum | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.182 | 4-nitrophenyl beta-D-galactoside + H2O | - |
Triticum aestivum | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.182 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Triticum aestivum | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.182 | 4-nitrophenyl beta-D-xyloside + H2O | - |
Triticum aestivum | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.182 | 7-methoxy-3-oxo-3,4-dihydro-2H-1,4 benzoxazin-2-yl beta-D-glucopyranoside + H2O | - |
Triticum aestivum | 2-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4 benzoxazin + beta-D-glucose | - |
? | |
| 3.2.1.182 | esculin + H2O | - |
Triticum aestivum | esculetin + beta-D-glucose | - |
? | |
| 3.2.1.182 | salicin + H2O | - |
Triticum aestivum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 58000-60000, SDS-PAGE | Triticum aestivum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.182 | beta-glucosidase | - |
Triticum aestivum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5.5 | - |
- |
Triticum aestivum |
| 3.2.1.182 | 5.5 | - |
- |
Triticum aestivum |
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