BRENDA - Enzyme Database

Characterization of a mutation in the IDH-II subunit of the NAD+-dependent isocitrate dehydrogenase from Arabidopsis thaliana

Lin, M.; Behal, R.H.; Oliver, D.J.; Plant Sci. 166, 983-988 (2004)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.41
gene idh-II
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.41
additional information
construction of a knockout mutant of subunit IDH-II, disruption by dissociation insertion element, mutant strain shows normal growth and development, but reduced enzyme activity in mitochondria compared to the wild-type plants, no altered phenotype of the mutant plants, but slightly reduced growth
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Arabidopsis thaliana
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Arabidopsis thaliana
key enzyme in the Krebs cycle
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Arabidopsis thaliana Landsberg
key enzyme in the Krebs cycle
2-oxoglutarate + CO2 + NADH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Arabidopsis thaliana
-
Landsberg
-
1.1.1.41
Arabidopsis thaliana Landsberg
-
Landsberg
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
mitochondrial preparation
Arabidopsis thaliana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
flower
-
Arabidopsis thaliana
-
1.1.1.41
leaf
-
Arabidopsis thaliana
-
1.1.1.41
plant
from liquid shaker cultures
Arabidopsis thaliana
-
1.1.1.41
root
-
Arabidopsis thaliana
-
1.1.1.41
silique
young
Arabidopsis thaliana
-
1.1.1.41
stem
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.41
0.092
-
partially purified enzyme, idh-II mutant
Arabidopsis thaliana
1.1.1.41
0.169
-
partially purified enzyme, wild-type
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
key enzyme in the Krebs cycle
657111
Arabidopsis thaliana
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
subunit IDH-I can substitute for a deficient mutant subunit IDH-II
657111
Arabidopsis thaliana
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
key enzyme in the Krebs cycle
657111
Arabidopsis thaliana Landsberg
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
subunit IDH-I can substitute for a deficient mutant subunit IDH-II
657111
Arabidopsis thaliana Landsberg
2-oxoglutarate + CO2 + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.41
More
subunit IDH-I can substitute for a deficient mutant subunit IDH-II
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.5
-
assay at
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
dependent on
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
gene idh-II
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
dependent on
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.41
additional information
construction of a knockout mutant of subunit IDH-II, disruption by dissociation insertion element, mutant strain shows normal growth and development, but reduced enzyme activity in mitochondria compared to the wild-type plants, no altered phenotype of the mutant plants, but slightly reduced growth
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Arabidopsis thaliana
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Arabidopsis thaliana
key enzyme in the Krebs cycle
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Arabidopsis thaliana Landsberg
key enzyme in the Krebs cycle
2-oxoglutarate + CO2 + NADH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
mitochondrial preparation
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
flower
-
Arabidopsis thaliana
-
1.1.1.41
leaf
-
Arabidopsis thaliana
-
1.1.1.41
plant
from liquid shaker cultures
Arabidopsis thaliana
-
1.1.1.41
root
-
Arabidopsis thaliana
-
1.1.1.41
silique
young
Arabidopsis thaliana
-
1.1.1.41
stem
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.41
0.092
-
partially purified enzyme, idh-II mutant
Arabidopsis thaliana
1.1.1.41
0.169
-
partially purified enzyme, wild-type
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
key enzyme in the Krebs cycle
657111
Arabidopsis thaliana
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
subunit IDH-I can substitute for a deficient mutant subunit IDH-II
657111
Arabidopsis thaliana
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
key enzyme in the Krebs cycle
657111
Arabidopsis thaliana Landsberg
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
subunit IDH-I can substitute for a deficient mutant subunit IDH-II
657111
Arabidopsis thaliana Landsberg
2-oxoglutarate + CO2 + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.41
More
subunit IDH-I can substitute for a deficient mutant subunit IDH-II
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.5
-
assay at
Arabidopsis thaliana