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Literature summary extracted from

  • Lin, M.; Behal, R.H.; Oliver, D.J.
    Characterization of a mutation in the IDH-II subunit of the NAD+-dependent isocitrate dehydrogenase from Arabidopsis thaliana (2004), Plant Sci., 166, 983-988.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 gene idh-II Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.41 additional information construction of a knockout mutant of subunit IDH-II, disruption by dissociation insertion element, mutant strain shows normal growth and development, but reduced enzyme activity in mitochondria compared to the wild-type plants, no altered phenotype of the mutant plants, but slightly reduced growth Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.41 mitochondrion
-
 Arabidopsis thaliana 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mg2+
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Arabidopsis thaliana key enzyme in the Krebs cycle 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ Arabidopsis thaliana Landsberg key enzyme in the Krebs cycle 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Arabidopsis thaliana
-
 Landsberg
-
1.1.1.41 Arabidopsis thaliana Landsberg
-
 Landsberg
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 mitochondrial preparation Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.41 flower
-
 Arabidopsis thaliana
-
1.1.1.41 leaf
-
 Arabidopsis thaliana
-
1.1.1.41 plant from liquid shaker cultures Arabidopsis thaliana
-
1.1.1.41 root
-
 Arabidopsis thaliana
-
1.1.1.41 silique young Arabidopsis thaliana
-
1.1.1.41 stem
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.41 0.092
-
 partially purified enzyme, idh-II mutant Arabidopsis thaliana
1.1.1.41 0.169
-
 partially purified enzyme, wild-type Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+ key enzyme in the Krebs cycle Arabidopsis thaliana 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ subunit IDH-I can substitute for a deficient mutant subunit IDH-II Arabidopsis thaliana 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ key enzyme in the Krebs cycle Arabidopsis thaliana Landsberg 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ subunit IDH-I can substitute for a deficient mutant subunit IDH-II Arabidopsis thaliana Landsberg 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 More subunit IDH-I can substitute for a deficient mutant subunit IDH-II Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 IDH
-
 Arabidopsis thaliana
1.1.1.41 NAD+-dependent isocitrate dehydrogenase
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.41 7.5
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+ dependent on Arabidopsis thaliana