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Literature summary extracted from

  • Lo Piero, A.R.; Cultrone, A.; Monachello, D.; Petrone, G.
    Different kinetic and regulatory properties of soluble and membrane-bound nitrate reductases in tomato leaves (2003), Plant Sci., 165, 139-145.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.1 MgCl2 0.1-5 mM, strong, both cytosolic and membrane-bound isozyme Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.035
-
 NADH pH 7.5, 30°C, membrane-bound isoform Solanum lycopersicum
1.7.1.1 0.048
-
 NADH pH 7.5, 30°C, cytosolic isoform Solanum lycopersicum
1.7.1.1 0.27
-
 nitrate pH 7.5, 30°C, cytosolic isoform Solanum lycopersicum
1.7.1.1 0.97
-
 nitrate pH 7.5, 30°C, membrane-bound isoform Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.1.1 cytosol two isozymes, a membranebound, and a cytosolic form Solanum lycopersicum 5829
-
1.7.1.1 membrane two isozymes, a membranebound, and a cytosolic form Solanum lycopersicum 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Solanum lycopersicum
-
 cv. Mill, membrane-bound isozyme remains more active in the dark and is more easily recovered after transfer to light than cytosolic isoform, inhibition of both forms by saline stress, cytosolic form is strongly induced and strictly dependent on presence of nitrate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1 extracting strength of different detergents on membrane-bound isozyme Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.1 leaf
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 NADH + nitrate + H+
-
 Solanum lycopersicum NAD+ + nitrite + H2O
-
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