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Literature summary extracted from

  • Santana, M.A.; Tan, F.C.; Smith, A.G.
    Molecular characterization of coproporphyrinogen oxidase from Glycine max and Arabidopsis thaliana (2002), Plant Physiol. Biochem., 40, 289-298.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.3 gene AtCPO-1, chromosome I, DNA and amino acid sequence determination and analysis, complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, expression as GFP-fusion protein in transgenic Arabidopsis thaliana plants via Agrobacterium tumefaciens tranbsformation Arabidopsis thaliana
1.3.3.3 gene AtCPO-2, DNA sequence determination and analysis, no complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, possibly a pseudogene Arabidopsis thaliana
1.3.3.3 over 200fold overexpression in Escherichia coli strain BL21(DE3), reduction of the toxicity of product protoporphyrin-IX for the bacterial cells by decrease of growth temperature to 20°C Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.3 0.00025
-
Coproporphyrinogen-III recombinant enzyme Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.3 chloroplast
-
Arabidopsis thaliana 9507
-
1.3.3.3 etioplast
-
Glycine max 9513
-
1.3.3.3 additional information no activity in mitochondria Glycine max
-
-
1.3.3.3 additional information no activity in mitochondrion and cytosol Arabidopsis thaliana
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.3 37000
-
x * 37000, SDS-PAGE Vigna radiata var. radiata
1.3.3.3 37000
-
x * 37000, SDS-PAGE Lotus japonicus
1.3.3.3 37000
-
2 * 37000, recombinant and native wild-type enzyme, SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.3 coproporphyrinogen-III + O2 Glycine max enzyme is involved in heme and chlorophyll biosynthesis protoporphyrinogen-IX + CO2 + H2O
-
?
1.3.3.3 coproporphyrinogen-III + O2 Arabidopsis thaliana enzyme is involved in heme and chlorophyll biosynthesis protoporphyrinogen-IX + CO2 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.3 Arabidopsis thaliana Q93Z96 2 genesAtCPO-1 and AtCPO-2, the latter being a pseudogene
-
1.3.3.3 Arabidopsis thaliana Q9LR75 2 genesAtCPO-1 and AtCPO-2, the latter being a pseudogene
-
1.3.3.3 Glycine max
-
-
-
1.3.3.3 Lotus japonicus
-
-
-
1.3.3.3 Vigna radiata var. radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.3 recombinant enzyme from Escherichia coli, 3-step procedure, 119fold to homogeneity Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.3 leaf
-
Vigna radiata var. radiata
-
1.3.3.3 leaf
-
Lotus japonicus
-
1.3.3.3 leaf
-
Arabidopsis thaliana
-
1.3.3.3 root
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.3 117.5
-
purified recombinant enzyme Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.3 coproporphyrinogen-III + O2
-
Glycine max protoporphyrinogen-IX + CO2 + H2O
-
?
1.3.3.3 coproporphyrinogen-III + O2
-
Arabidopsis thaliana protoporphyrinogen-IX + CO2 + H2O
-
?
1.3.3.3 coproporphyrinogen-III + O2 enzyme is involved in heme and chlorophyll biosynthesis Glycine max protoporphyrinogen-IX + CO2 + H2O
-
?
1.3.3.3 coproporphyrinogen-III + O2 enzyme is involved in heme and chlorophyll biosynthesis Arabidopsis thaliana protoporphyrinogen-IX + CO2 + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.3.3.3 ? x * 37000, SDS-PAGE Vigna radiata var. radiata
1.3.3.3 ? x * 37000, SDS-PAGE Lotus japonicus
1.3.3.3 dimer 2 * 37000, recombinant and native wild-type enzyme, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
1.3.3.3 coproporphyrinogen III oxidase
-
Vigna radiata var. radiata
1.3.3.3 coproporphyrinogen III oxidase
-
Glycine max
1.3.3.3 coproporphyrinogen III oxidase
-
Lotus japonicus
1.3.3.3 coproporphyrinogen III oxidase
-
Arabidopsis thaliana
1.3.3.3 CPO
-
Vigna radiata var. radiata
1.3.3.3 CPO
-
Glycine max
1.3.3.3 CPO
-
Lotus japonicus
1.3.3.3 CPO
-
Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.3 30
-
assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.3 8
-
assay at Arabidopsis thaliana