EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.3.3 | gene AtCPO-1, chromosome I, DNA and amino acid sequence determination and analysis, complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, expression as GFP-fusion protein in transgenic Arabidopsis thaliana plants via Agrobacterium tumefaciens tranbsformation | Arabidopsis thaliana |
1.3.3.3 | gene AtCPO-2, DNA sequence determination and analysis, no complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, possibly a pseudogene | Arabidopsis thaliana |
1.3.3.3 | over 200fold overexpression in Escherichia coli strain BL21(DE3), reduction of the toxicity of product protoporphyrin-IX for the bacterial cells by decrease of growth temperature to 20°C | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.3.3 | 0.00025 | - |
Coproporphyrinogen-III | recombinant enzyme | Glycine max |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.3.3 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
1.3.3.3 | etioplast | - |
Glycine max | 9513 | - |
1.3.3.3 | additional information | no activity in mitochondria | Glycine max | - |
- |
1.3.3.3 | additional information | no activity in mitochondrion and cytosol | Arabidopsis thaliana | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.3.3 | 37000 | - |
x * 37000, SDS-PAGE | Vigna radiata var. radiata |
1.3.3.3 | 37000 | - |
x * 37000, SDS-PAGE | Lotus japonicus |
1.3.3.3 | 37000 | - |
2 * 37000, recombinant and native wild-type enzyme, SDS-PAGE | Glycine max |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.3.3 | coproporphyrinogen-III + O2 | Glycine max | enzyme is involved in heme and chlorophyll biosynthesis | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
1.3.3.3 | coproporphyrinogen-III + O2 | Arabidopsis thaliana | enzyme is involved in heme and chlorophyll biosynthesis | protoporphyrinogen-IX + CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.3.3 | Arabidopsis thaliana | Q93Z96 | 2 genesAtCPO-1 and AtCPO-2, the latter being a pseudogene | - |
1.3.3.3 | Arabidopsis thaliana | Q9LR75 | 2 genesAtCPO-1 and AtCPO-2, the latter being a pseudogene | - |
1.3.3.3 | Glycine max | - |
- |
- |
1.3.3.3 | Lotus japonicus | - |
- |
- |
1.3.3.3 | Vigna radiata var. radiata | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.3.3 | recombinant enzyme from Escherichia coli, 3-step procedure, 119fold to homogeneity | Glycine max |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.3.3 | leaf | - |
Vigna radiata var. radiata | - |
1.3.3.3 | leaf | - |
Lotus japonicus | - |
1.3.3.3 | leaf | - |
Arabidopsis thaliana | - |
1.3.3.3 | root | - |
Arabidopsis thaliana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.3.3 | 117.5 | - |
purified recombinant enzyme | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.3.3 | coproporphyrinogen-III + O2 | - |
Glycine max | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
1.3.3.3 | coproporphyrinogen-III + O2 | - |
Arabidopsis thaliana | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
1.3.3.3 | coproporphyrinogen-III + O2 | enzyme is involved in heme and chlorophyll biosynthesis | Glycine max | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
1.3.3.3 | coproporphyrinogen-III + O2 | enzyme is involved in heme and chlorophyll biosynthesis | Arabidopsis thaliana | protoporphyrinogen-IX + CO2 + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.3.3 | ? | x * 37000, SDS-PAGE | Vigna radiata var. radiata |
1.3.3.3 | ? | x * 37000, SDS-PAGE | Lotus japonicus |
1.3.3.3 | dimer | 2 * 37000, recombinant and native wild-type enzyme, SDS-PAGE | Glycine max |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.3.3 | coproporphyrinogen III oxidase | - |
Vigna radiata var. radiata |
1.3.3.3 | coproporphyrinogen III oxidase | - |
Glycine max |
1.3.3.3 | coproporphyrinogen III oxidase | - |
Lotus japonicus |
1.3.3.3 | coproporphyrinogen III oxidase | - |
Arabidopsis thaliana |
1.3.3.3 | CPO | - |
Vigna radiata var. radiata |
1.3.3.3 | CPO | - |
Glycine max |
1.3.3.3 | CPO | - |
Lotus japonicus |
1.3.3.3 | CPO | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.3.3 | 30 | - |
assay at | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.3.3 | 8 | - |
assay at | Arabidopsis thaliana |