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Literature summary extracted from

  • Hornberger, M.; Bottigheimer, U.; Hillier-Kaiser, A.; Kreis, W.
    Purification and characterization of the cardenolide-specific beta-glucohydrolase CGH II from Digitalis lanata leaves (2000), Plant Physiol. Biochem., 38, 929-936.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Phenylmercuriacetate 0.1 mM, 80% inhibition, 1 mM, complete inhibition, activity is restored by addition of 10 mM beta-mercaptoethanol Digitalis lanata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 65000
-
 1 * 65000, SDS-PAGE Digitalis lanata
3.2.1.21 70000
-
 gel filtration Digitalis lanata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 digitoxigenin cellobioside + 2 H2O Digitalis lanata 38% of activity with glucoevatromonoside digitoxigenin + 2 beta-D-glucose
-
 ?
3.2.1.21 glucoevatromonoside + H2O Digitalis lanata
-
 evatromonoside + beta-D-glucose
-
 ?
3.2.1.21 glucogitoroside + H2O Digitalis lanata 38% of activity with glucoevatromonoside gitoroside + beta-D-glucose
-
 ?
3.2.1.21 glucoquinovosidodigitoxigenin + H2O Digitalis lanata 38% of activity with glucoevatromonoside quinovosidodigitoxigenin + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Digitalis lanata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 ammonium sulfate, Source Q, Macrosep, Superdex 200, hydroxyapatite Digitalis lanata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 leaf
-
 Digitalis lanata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 digitoxigenin cellobioside + 2 H2O 38% of activity with glucoevatromonoside Digitalis lanata digitoxigenin + 2 beta-D-glucose
-
 ?
3.2.1.21 glucoevatromonoside + H2O
-
 Digitalis lanata evatromonoside + beta-D-glucose
-
 ?
3.2.1.21 glucogitoroside + H2O 38% of activity with glucoevatromonoside Digitalis lanata gitoroside + beta-D-glucose
-
 ?
3.2.1.21 glucoquinovosidodigitoxigenin + H2O 38% of activity with glucoevatromonoside Digitalis lanata quinovosidodigitoxigenin + beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Digitalis lanata p-nitrophenol + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 65000, SDS-PAGE Digitalis lanata

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 CGHII
-
 Digitalis lanata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Digitalis lanata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 35 60 50% of maximal activity at 35°C and 55°C, no activity above 60°C Digitalis lanata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
-
 Digitalis lanata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 4.6 7 50% of maximal activity at pH 5.0 and pH 7.0, respectively Digitalis lanata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Digitalis lanata isoelectric focusing
-
 6