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Literature summary extracted from

  • Lancaster, J.E.; Shaw, M.L.; Joyce, M.D.; McCallum, J.A.; McManus, M.T.
    A novel alliinase from onion roots. Biochemical characterization and cDNA cloning (2000), Plant Physiol., 122, 1269-1279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.4
-
 Allium cepa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.4 2.2
-
 cystine alliinase I Allium cepa
4.4.1.4 2.6
-
 S-propenyl-L-Cys sulfoxide alliinase I Allium cepa
4.4.1.4 3
-
 S-propenyl-L-Cys sulfoxide alliinase II Allium cepa
4.4.1.4 9.7
-
 cystine alliinase II Allium cepa
4.4.1.4 16.3
-
 S-propyl-L-Cys sulfoxide alliinase II Allium cepa
4.4.1.4 18.3
-
 S-allyl-L-Cys sulfoxide alliinase I Allium cepa
4.4.1.4 22
-
 S-propyl-L-Cys sulfoxide alliinase I Allium cepa
4.4.1.4 24.1
-
 S-allyl-L-Cys sulfoxide alliinase II Allium cepa
4.4.1.4 45.7
-
 S-ethyl-L-Cys sulfoxide alliinase I Allium cepa
4.4.1.4 47
-
 S-methyl-L-Cys sulfoxide alliinase I Allium cepa
4.4.1.4 68.4
-
 S-ethyl-L-Cys sulfoxide alliinase II Allium cepa
4.4.1.4 96.6
-
 S-methyl-L-Cys sulfoxide alliinase II Allium cepa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.4 51000
-
 alliinase I, gel filtration Allium cepa
4.4.1.4 52700
-
 1 * 52700, alliinase I, SDS-PAGE Allium cepa
4.4.1.4 57500
-
 alliinase II, gel filtration Allium cepa

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.4 Allium cepa
-
-
-
4.4.1.4 Leucocoryne angustipetala
-
-
-
4.4.1.4 Leucocoryne coquimbensis
-
-
-
4.4.1.4 Leucocoryne odorata
-
-
-
4.4.1.4 Leucocoryne purpurea
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.4.1.4 glycoprotein alliinase I and alliinase II differ in glycosylation. Both contain xylose/fucose containing complex-type N-linked glycans, and alliinase II also contains terminal mannose structures Allium cepa

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.4
-
 Allium cepa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.4 bulb
-
 Leucocoryne angustipetala
-
4.4.1.4 bulb
-
 Leucocoryne purpurea
-
4.4.1.4 bulb only in Leucocoryne odorata
-
4.4.1.4 leaf
-
 Leucocoryne angustipetala
-
4.4.1.4 leaf
-
 Leucocoryne purpurea
-
4.4.1.4 leaf only in Allium cepa
-
4.4.1.4 leaf only in Leucocoryne coquimbensis
-
4.4.1.4 root
-
 Allium cepa
-
4.4.1.4 scape
-
 Leucocoryne angustipetala
-
4.4.1.4 scape
-
 Leucocoryne purpurea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.4 119.7
-
-
 Allium cepa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.4 (+)-S-propyl-L-cysteine sulfoxide
-
 Allium cepa propane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 (+)-S-propyl-L-cysteine sulfoxide
-
 Leucocoryne angustipetala propane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 (+)-S-propyl-L-cysteine sulfoxide
-
 Leucocoryne purpurea propane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 (+)-S-propyl-L-cysteine sulfoxide
-
 Leucocoryne coquimbensis propane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 (+)-S-propyl-L-cysteine sulfoxide
-
 Leucocoryne odorata propane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 cystine
-
 Allium cepa ?
-
 ?
4.4.1.4 S-allyl-L-Cys sulfoxide + H2O
-
 Allium cepa 2-propenesulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 S-ethyl-L-Cys sulfoxide + H2O
-
 Allium cepa ethanesulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 S-methyl-L-Cys sulfoxide
-
 Allium cepa methanesulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 S-propenyl-L-Cys sulfoxide
-
 Allium cepa ?
-
 ?
4.4.1.4 S-propyl-L-Cys sulfoxide
-
 Allium cepa propanesulfenic acid + pyruvate + NH4+
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.4 monomer 1 * 52700, alliinase I, SDS-PAGE Allium cepa
4.4.1.4 monomer 1 * 57500, alliinase II, SDS-PAGE Allium cepa

Synonyms

EC Number Synonyms Comment Organism
4.4.1.4 alliinase I
-
 Allium cepa
4.4.1.4 alliinase II
-
 Allium cepa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.4.1.4 Allium cepa alliinase I, isoelectric focusing 4.7 4.6
4.4.1.4 Allium cepa alliinase II, isoelectric focusing
-
 6.9