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Literature summary extracted from

  • Van den Ende, W.; De Coninck, B.; Clerens, S.; Vergauwen, R.; Van Laere, A.
    Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.) (2003), Plant J., 36, 697-710.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.65 expression in Pichia pastoris Beta vulgaris
3.2.1.80 expression in Pichia pastoris Beta vulgaris
3.2.1.154 heterologous expression in Pichia pastoris Beta vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.154 sucrose
-
 Beta vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.154 77
-
 6-kestose
-
 Beta vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.65 cell wall
-
 Beta vulgaris 5618
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.65 Beta vulgaris
-
-
-
3.2.1.80 Beta vulgaris
-
 sugar beet
-
3.2.1.154 Beta vulgaris
-
 cv. Opus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80
-
 Beta vulgaris
3.2.1.154
-
 Beta vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.65 root
-
 Beta vulgaris
-
3.2.1.80 root
-
 Beta vulgaris
-
3.2.1.154 leaf
-
 Beta vulgaris
-
3.2.1.154 petiole
-
 Beta vulgaris
-
3.2.1.154 root
-
 Beta vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.154 12.5
-
-
 Beta vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.65 levan-type fructans (beta-2,6)
-
 Beta vulgaris D-fructose
-
 ?
3.2.1.80 inulin-type fructans (beta-2,1) + H2O poorly substrate Beta vulgaris D-fructose
-
 ?
3.2.1.80 levan-type fructans (beta-2,6) + H2O best substrate Beta vulgaris D-fructose
-
 ?
3.2.1.154 1-kestose + H2O native enzyme shows 12% of the activity with levan Beta vulgaris ?
-
 ?
3.2.1.154 6-kestose + H2O native enzyme shows 77% of the activity with levan Beta vulgaris ?
-
 ?
3.2.1.154 levan + H2O
-
 Beta vulgaris ?
-
 ?
3.2.1.154 levanbiose + H2O native enzyme shows 63% of the activity with levan Beta vulgaris beta-D-fructose
-
 ?
3.2.1.154 additional information no activity with sucrose, 1,1-nystose and inulin Beta vulgaris ?
-
 ?
3.2.1.154 neokestin + H2O native enzyme shows 25% of the activity with levan Beta vulgaris ?
-
 ?
3.2.1.154 neokestose + H2O native enzyme shows 93% of the activity with levan Beta vulgaris ?
-
 ?
3.2.1.154 phlein + H2O native enzyme shows 84% of the activity with levan Beta vulgaris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.65 6-FEHs
-
 Beta vulgaris
3.2.1.65 fructan-6-exohydrolase
-
 Beta vulgaris
3.2.1.80 6-FEH
-
 Beta vulgaris
3.2.1.80 fructan 6-exohydrolase
-
 Beta vulgaris
3.2.1.80 levanase
-
 Beta vulgaris
3.2.1.154 6-FEH
-
 Beta vulgaris
3.2.1.154 fructan 6-exohydrolase
-
 Beta vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.154 30
-
-
 Beta vulgaris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.154 18 38 18°C: about 45% of maximal activity, 38°C: about 20% of maximal activity Beta vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.154 5
-
-
 Beta vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.154 4.3 6.8 pH 4.3: about 65% of maximal activity, pH 6.8: about 40% of maximal activity Beta vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.154 134
-
 sucrose
-
 Beta vulgaris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.80 Beta vulgaris
-
-
 additional information