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Literature summary extracted from

  • Suzuki, H.; Sawada, S.; Yonekura-Sakakibara, K.; Nakayama, T.; Yamaguchi, M.; Nishino, T.
    Identification of a cDNA encoding malonyl-coenzyme A:anthocyanidin 3-O-glucoside 6''-O-malonyltransferase from cineraria (Senecio cruentus) flowers (2003), Plant Biotechnol., 20, 229-234.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.3.1.171 agriculture the enzyme may serve as a tool for the metabolic engineering of flavonoid biosynthesis leading to the accumulation of cinerarin in flowers, which results in the coloration of blue flowers Pericallis cruenta

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.171 expression in Escherichia coli JM109 under the control of T5 promoter as an in-frame N-terminal fusion with a His6 tag Pericallis cruenta

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.171 acetyl-CoA 0.1 mM, 63% inhibition Pericallis cruenta
2.3.1.171 CdCl2 0.1 mM, 48% loss of activity Pericallis cruenta
2.3.1.171 CoA 0.1 mM, 67% inhibition Pericallis cruenta
2.3.1.171 CuCl2 0.1 mM, complete inhibition Pericallis cruenta
2.3.1.171 HgCl2 0.1 mM, complete inhibition Pericallis cruenta
2.3.1.171 MgCl2 0.1 mM, 52% inhibition Pericallis cruenta
2.3.1.171 additional information 0.1 mM EDTA or 0.1 mM CaCl2, CoCl2, FeCl2, MnCl2, NiCl2 or SnCl2 have negligible effects on the enzyme activity Pericallis cruenta
2.3.1.171 NEM 5 mM, complete inhibition Pericallis cruenta
2.3.1.171 ZnCl2 0.1 mM, 66% inhibition Pericallis cruenta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.171 0.011
-
 pelargonidin 3-O-beta-D-glucoside
-
 Pericallis cruenta
2.3.1.171 0.061
-
 malonyl-CoA
-
 Pericallis cruenta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.171 malonyl-CoA + delphinidin 3-O-beta-D-glucoside Pericallis cruenta because the reaction product delphinidin 3-O-6'‘-malonylglucoside, is a part of cinerarin biosynthesis, it is highly likely that Sc3MaT is involved in the biosynthesis of cinerarin CoA + delphinidin 3-O-(6-malonyl-beta-D-glucoside)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.171 Pericallis cruenta
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.171 flower
-
 Pericallis cruenta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.171 malonyl-CoA + cyanidin 3-O-beta-D-glucoside 110% of the activity with pelargonidin 3-O-glucoside Pericallis cruenta CoA + cyanidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
 ?
2.3.1.171 malonyl-CoA + delphinidin 3-O-beta-D-glucoside because the reaction product delphinidin 3-O-6'‘-malonylglucoside, is a part of cinerarin biosynthesis, it is highly likely that Sc3MaT is involved in the biosynthesis of cinerarin Pericallis cruenta CoA + delphinidin 3-O-(6-malonyl-beta-D-glucoside)
-
 ?
2.3.1.171 malonyl-CoA + delphinidin 3-O-beta-D-glucoside 153% of the activity with pelargonidin 3-O-glucoside Pericallis cruenta CoA + delphinidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
 ?
2.3.1.171 malonyl-CoA + pelargonidin 3-O-beta-D-glucoside
-
 Pericallis cruenta CoA + pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
 ?
2.3.1.171 methylmalonyl-CoA + pelargonidin 3-O-beta-D-glucoside 15% of the activity with methylmalonyl-CoA Pericallis cruenta CoA + pelargonidin 3-O-(6-O-methylmalonyl-beta-D-glucoside)
-
 ?
2.3.1.171 succinyl-CoA + pelargonidin 3-O-beta-D-glucoside 20% of the activity with methylmalonyl-CoA Pericallis cruenta CoA + pelargonidin 3-O-(6-O-succinyl-beta-D-glucoside)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.171 3MaT
-
 Pericallis cruenta
2.3.1.171 anthocyanidin 3-glucoside malonyltransferase
-
 Pericallis cruenta
2.3.1.171 anthocyanin 3-glucoside malonyltransferase
-
 Pericallis cruenta
2.3.1.171 Dv3MaT
-
 Pericallis cruenta
2.3.1.171 malonyl-CoA:anthocyanin 3-O-glucoside-6'‘-O-malonyltransferase
-
 Pericallis cruenta
2.3.1.171 malonyl-coenzyme A:anthocyanidin 3-O-glucoside 6''-O-malonyltransferase
-
 Pericallis cruenta
2.3.1.171 malonyl-coenzyme A:anthocyanidin-3-O-beta-D-glucoside 6''-O-malonyltransferase
-
 Pericallis cruenta
2.3.1.171 malonyltransferase, anthocyanidin 3-glucoside
-
 Pericallis cruenta
2.3.1.171 Sc3MaT
-
 Pericallis cruenta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.171 30
-
 pH 7.0, 20 min, stable below Pericallis cruenta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.171 8.8
-
 malonyl-CoA
-
 Pericallis cruenta
2.3.1.171 8.8
-
 pelargonidin 3-O-beta-D-glucoside
-
 Pericallis cruenta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.171 7.5
-
-
 Pericallis cruenta

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.171 5.5 7.5 pH 7.0, 20 min, stable Pericallis cruenta