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Literature summary extracted from

  • Trail, F.; Xu, H.
    Purification and characterization of mannitol dehydrogenase and identification of the corresponding cDNA from the head blight fungus, Gibberella zeae (Fusarium graminearum) (2002), Phytochemistry, 61, 791-796.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.138 D-fructose inhibitory above 600 mM Fusarium graminearum
1.1.1.138 NADPH inhibitory above 0.3 mM Fusarium graminearum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 0.07
-
 NADPH cosubstrate fructose, pH 7.0, 30°C Fusarium graminearum
1.1.1.138 0.078
-
 NADP+ cosubstrate mannitol, pH 9.0, 30°C Fusarium graminearum
1.1.1.138 0.14
-
 NADP+ cosubstrate mannitol, pH 7.0, 30°C Fusarium graminearum
1.1.1.138 40
-
 D-mannitol cosubstrate NADP+, pH 9.0, 30°C Fusarium graminearum
1.1.1.138 57
-
 D-mannitol cosubstrate NADP+, pH 7.0, 30°C Fusarium graminearum
1.1.1.138 290
-
 D-fructose cosubstrate NADPH, pH 7.0, 30°C Fusarium graminearum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.138 29000
-
 4 * 29000, SDS-PAGE Fusarium graminearum
1.1.1.138 110000
-
 gel filtration Fusarium graminearum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Fusarium graminearum
-
 Fusarium graminearum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.138
-
 Fusarium graminearum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 0.54
-
 pH 9.0, 30°C Fusarium graminearum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 D-fructose + NADPH
-
 Fusarium graminearum D-mannitol + NADP+
-
 r
1.1.1.138 D-mannitol + NADP+
-
 Fusarium graminearum D-fructose + NADPH + H+
-
 r
1.1.1.138 L-sorbitol + NADP+ 21% of the activity with D-mannitol Fusarium graminearum L-sorbose + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.138 tetramer 4 * 29000, SDS-PAGE Fusarium graminearum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 7
-
 D-fructose reduction Fusarium graminearum
1.1.1.138 9
-
 D-mannitol oxidation Fusarium graminearum