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Literature summary extracted from

  • Kim, W.D.; Kobayashi, O.; Kaneko, S.; Sakakibara, Y.; Park, G.G.; Kusakabe, I.; Tanaka, H.; Kobayashi, H.
    alpha-Galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells (2002), Phytochemistry, 61, 621-630.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22
-
 Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag2+ 1 mM, 46% inhibition Oryza sativa
3.2.1.22 Hg2+ 1 mM, 98% inhibition Oryza sativa
3.2.1.22 PCMB 1 mM, 98% inhibition Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 39950
-
 1 * 39950, calculation from nucleotide sequence Oryza sativa
3.2.1.22 40000
-
 gel filtration Oryza sativa
3.2.1.22 41000
-
 1 * 41000, SDS-PAGE Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Oryza sativa Q9FXT4 L. var. Nipponbare
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 cell suspension culture
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 119.7
-
-
 Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 alpha-D-galactopyranosyl-(1-6)-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
-
 Oryza sativa alpha-D-galactose + beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose
-
 ?
3.2.1.22 melibiose + H2O
-
 Oryza sativa alpha-D-galactose + alpha-D-glucose
-
 ?
3.2.1.22 O-beta-D-mannopyranosyl-(1-4)-O-[alpha-D-galactopyranosyl-(1-6)]-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
-
 Oryza sativa ?
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Oryza sativa p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Oryza sativa sucrose + alpha-D-galactose
-
 ?
3.2.1.22 stachyose + 2 H2O
-
 Oryza sativa 2 alpha-D-galactose + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 monomer 1 * 41000, SDS-PAGE Oryza sativa
3.2.1.22 monomer 1 * 39950, calculation from nucleotide sequence Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 45
-
 activity with p-nitrophenyl alpha-D-galactopyranoside Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 40
-
 inactivation above Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5
-
-
 Oryza sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 3 8 inactivation below pH 3 and above pH 8 Oryza sativa