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Literature summary extracted from
- Invertase from a strain of Rhodotorula glutinis (2002), Phytochemistry, 61, 605-609.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.26 | 227 | - |
sucrose | pH 4.5, 35°C | Rhodotorula glutinis |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.26 | Ca2+ | 5 mM, 3.4fold stimulation | Rhodotorula glutinis | |
| 3.2.1.26 | Co2+ | 5 mM, 1.7fold stimulation | Rhodotorula glutinis | |
| 3.2.1.26 | Cu2+ | 5 mM, 2fold stimulation | Rhodotorula glutinis | |
| 3.2.1.26 | Fe2+ | 5 mM, 2.4fold stimulation | Rhodotorula glutinis | |
| 3.2.1.26 | Mg2+ | 5 mM, 3.5fold stimulation | Rhodotorula glutinis | |
| 3.2.1.26 | Na+ | 5 mM, 2fold stimulation | Rhodotorula glutinis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 47000 | - |
2 * 47000, SDS-PAGE | Rhodotorula glutinis |
| 3.2.1.26 | 100000 | - |
gel filtration | Rhodotorula glutinis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.26 | Rhodotorula glutinis | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.26 | glycoprotein | contains 19% carbohydrate | Rhodotorula glutinis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.26 | - |
Rhodotorula glutinis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 12 | - |
- |
Rhodotorula glutinis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.26 | additional information | no transfructosylation reraction at 1 M sucrose | Rhodotorula glutinis | ? | - |
? | |
| 3.2.1.26 | raffinose + H2O | - |
Rhodotorula glutinis | beta-D-fructose + melibiose | - |
? | |
| 3.2.1.26 | stachyose + H2O | - |
Rhodotorula glutinis | ? | - |
? | |
| 3.2.1.26 | sucrose + H2O | - |
Rhodotorula glutinis | beta-D-fructose + alpha-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.26 | dimer | 2 * 47000, SDS-PAGE | Rhodotorula glutinis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 60 | - |
half-life is 30 min | Rhodotorula glutinis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 4.5 | - |
- |
Rhodotorula glutinis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 4 | 5 | pH 4: about 70% of maximal activity, pH 5.0: about 60% of maximal activity | Rhodotorula glutinis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 2.6 | 5.5 | stable | Rhodotorula glutinis |
| 3.2.1.26 | 6 | - |
30 min, 10% loss of activity | Rhodotorula glutinis |
| 3.2.1.26 | 8 | - |
30 min, 93% loss of activity | Rhodotorula glutinis |
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