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Literature summary extracted from

  • Guimaraes, V.M.; Tavares de Rezende, S.; Moreira, M.A.; Goncalves de Barros, E.; Felix, C.R.
    Characterization of alpha-galactosidases from germinating soybean seed and their use for hydrolysis of oligosaccharides (2001), Phytochemistry, 58, 67-73.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 alpha-D-galactose 2 mM, 17% inhibition of enzyme P1, 68.5% inhibition of enzyme P2 Glycine max
3.2.1.22 CuSO4 2 mM, 94.5% inhibition of enzyme P1, 48% inhibition of enzyme P2 Glycine max
3.2.1.22 galactose
-
 Glycine max
3.2.1.22 melibiose 2 mM, 16% inhibition of enzyme P1, 36% inhibition of enzyme P2 Glycine max
3.2.1.22 SDS 1 mM, 98% inhibition of enzyme P1, 37% inhibition of enzyme P2 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.76
-
 p-nitrophenyl alpha-D-galactopyranoside enzyme form P2 Glycine max
3.2.1.22 1.55
-
 p-nitrophenyl alpha-D-galactopyranoside enzyme form P1 Glycine max
3.2.1.22 5.34
-
 raffinose enzyme form P2 Glycine max
3.2.1.22 5.53
-
 melibiose enzyme form P2 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 33000
-
 x * 33000, enzyme form P1, SDS-PAGE Glycine max

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 one of the enzyme preparations, P1, shows a single protein with 33000 Da and the second preparation, P2, has two proteins with 31000 Da and 33000 Da Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 seed germinating Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 14.2
-
 enzyme form P1 Glycine max
3.2.1.22 62.5
-
 enzyme form P2 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 lactose + H2O enzyme P1: 6.2% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max beta-D-galactose + D-glucose
-
 ?
3.2.1.22 maltose + H2O enzyme P1: 4% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max alpha-D-glucose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O enzyme P1: 6.9% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max alpha-D-galactose + D-glucose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Glycine max p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl beta-D-galactopyranoside + H2O enzyme P1: 5.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: no activity Glycine max p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O enzyme P1: 11.8% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 54.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max sucrose + alpha-D-galactose
-
 ?
3.2.1.22 stachyose + 2 H2O enzyme P1: 5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max 2 alpha-D-galactose + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 ? x * 33000, enzyme form P1, SDS-PAGE Glycine max
3.2.1.22 More one of the enzyme preparations, P1, shows a single protein with 33000 Da and the second preparation, P2, has two proteins with 31000 Da and 33000 Da Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 45
-
 enzyme form P1 Glycine max
3.2.1.22 50
-
 enzyme form P2 Glycine max

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.22 30 70 about 50% of maximal activity at 30°C and at 70°C, enzyme P1 Glycine max
3.2.1.22 35 55 35°C: about 40% of maximal activity, 55°C: about 40% of maximal activity, enzyme P2 Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 40
-
 2 h, both enzymes retain about 50% of their original activity Glycine max
3.2.1.22 50
-
 45 min, 90% loss of activity of enzyme P1 and P2 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5
-
 enzyme form P1 Glycine max
3.2.1.22 5.5
-
 enzyme form P2 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 3 6.5 pH 3.0: enzyme P1 shows about 60% of maximal activity, enzyme P2 shows about 70% of maximal activity, pH 6.5: enzyme p1 shows about 30% of maximal activity, enzyme P2 shows about 75% of maximal activity Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.22 0.65
-
 galactose enzyme form P2 Glycine max