BRENDA - Enzyme Database

Barley glutamyl tRNAGlu reductase: mutations affecting haem inhibition and enzyme activity

Vothknecht, U.C.; Kannangara, C.G.; von Wettstein, D.; Phytochemistry 47, 513-519 (1998)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.70
I318L/R322G/N454D
mutant enzyme with greatly reduced activity
Hordeum vulgare
1.2.1.70
I464P
mutant enzyme with greatly reduced activity
Hordeum vulgare
1.2.1.70
L387H/L302S
mutant enzyme with greatly reduced activity
Hordeum vulgare
1.2.1.70
M122K/K154N/F371L/E400K
mutant enzyme with greatly reduced activity
Hordeum vulgare
1.2.1.70
additional information
a 30 amino acid N-terminal deletion has no detrimental effect on the catalytic activity of the enzyme
Hordeum vulgare
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
heme
0.002 mM, 63% inhibition of non-truncated enzyme
Hordeum vulgare
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Hordeum vulgare
-
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Hordeum vulgare
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
656936
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Hordeum vulgare
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Hordeum vulgare
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.70
I318L/R322G/N454D
mutant enzyme with greatly reduced activity
Hordeum vulgare
1.2.1.70
I464P
mutant enzyme with greatly reduced activity
Hordeum vulgare
1.2.1.70
L387H/L302S
mutant enzyme with greatly reduced activity
Hordeum vulgare
1.2.1.70
M122K/K154N/F371L/E400K
mutant enzyme with greatly reduced activity
Hordeum vulgare
1.2.1.70
additional information
a 30 amino acid N-terminal deletion has no detrimental effect on the catalytic activity of the enzyme
Hordeum vulgare
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
heme
0.002 mM, 63% inhibition of non-truncated enzyme
Hordeum vulgare
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Hordeum vulgare
-
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
656936
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?