BRENDA - Enzyme Database

Malonyl-coenzyme A:anthocyanidin 3-glucoside malonyltransferase from flowers of Callistephus chinensis

Teusch, M.; Forkmann, G.; Phytochemistry 26, 2181-2183 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.171
CaCl2
1 mM, 20% inhibition
Callistephus chinensis
2.3.1.171
CoCl2
1 mM, 9% inhibition
Callistephus chinensis
2.3.1.171
CuSO4
1 mM, 80% inhibition
Callistephus chinensis
2.3.1.171
diethyldicarbonate
1 mM, 45% inhibition
Callistephus chinensis
2.3.1.171
diethyldithiocarbamate
2 mM, 36% inhibition
Callistephus chinensis
2.3.1.171
EDTA
2 mM, 34% inhibition
Callistephus chinensis
2.3.1.171
FeSO4
1 mM, 45% inhibition
Callistephus chinensis
2.3.1.171
KCN
1 mM, 26% inhibition
Callistephus chinensis
2.3.1.171
MgCl2
1 mM, 14% inhibition
Callistephus chinensis
2.3.1.171
MnCl2
1 mM, 28% inhibition
Callistephus chinensis
2.3.1.171
PCMB
0.2 mM, 64% inhibition
Callistephus chinensis
2.3.1.171
ZnCl2
1 mM, 37% inhibition
Callistephus chinensis
2.3.1.171
ZnSO4
1 mM, 46% inhibition
Callistephus chinensis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.171
Callistephus chinensis
-
-
-
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.171
-20°C, enzyme preparation can be stored for several weeks without appreciable loss of activity
Callistephus chinensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.171
glutaryl-CoA + pelargonidin 3-O-beta-D-glucoside
50% of the activity with malonyl-CoA
656933
Callistephus chinensis
CoA + pelargonidin 3-O-(6-O-glutaryl-beta-D-glucoside)
-
-
-
?
2.3.1.171
malonyl-CoA + cyanidin 3-O-beta-D-glucoside
-
656933
Callistephus chinensis
CoA + cyanidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
-
-
?
2.3.1.171
malonyl-CoA + delphinidin 3,5-di-O-beta-D-glucoside
10% of the activity with pelargonidin 3-O-glucoside
656933
Callistephus chinensis
CoA + ?
-
-
-
?
2.3.1.171
malonyl-CoA + delphinidin 3-O-beta-D-glucoside
-
656933
Callistephus chinensis
CoA + delphinidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
-
-
?
2.3.1.171
malonyl-CoA + pelargonidin 3-O-beta-D-glucoside
-
656933
Callistephus chinensis
CoA + pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
-
-
?
2.3.1.171
methylmalonyl-CoA + pelargonidin 3-O-beta-D-glucoside
30% of the activity with malonyl-CoA
656933
Callistephus chinensis
CoA + pelargonidin 3-O-(6-O-methylmalonyl-beta-D-glucoside)
-
-
-
?
2.3.1.171
succinyl-CoA + pelargonidin 3-O-beta-D-glucoside
40% of the activity with malonyl-CoA
656933
Callistephus chinensis
CoA + pelargonidin 3-O-(6-O-succinyl-beta-D-glucoside)
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.171
50
-
-
Callistephus chinensis
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.1.171
30
80
50% of maximal activity at 30°C and at 80°C, 0°C: 17% of maximal activity activity
Callistephus chinensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.171
7
-
-
Callistephus chinensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.171
CaCl2
1 mM, 20% inhibition
Callistephus chinensis
2.3.1.171
CoCl2
1 mM, 9% inhibition
Callistephus chinensis
2.3.1.171
CuSO4
1 mM, 80% inhibition
Callistephus chinensis
2.3.1.171
diethyldicarbonate
1 mM, 45% inhibition
Callistephus chinensis
2.3.1.171
diethyldithiocarbamate
2 mM, 36% inhibition
Callistephus chinensis
2.3.1.171
EDTA
2 mM, 34% inhibition
Callistephus chinensis
2.3.1.171
FeSO4
1 mM, 45% inhibition
Callistephus chinensis
2.3.1.171
KCN
1 mM, 26% inhibition
Callistephus chinensis
2.3.1.171
MgCl2
1 mM, 14% inhibition
Callistephus chinensis
2.3.1.171
MnCl2
1 mM, 28% inhibition
Callistephus chinensis
2.3.1.171
PCMB
0.2 mM, 64% inhibition
Callistephus chinensis
2.3.1.171
ZnCl2
1 mM, 37% inhibition
Callistephus chinensis
2.3.1.171
ZnSO4
1 mM, 46% inhibition
Callistephus chinensis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.171
-20°C, enzyme preparation can be stored for several weeks without appreciable loss of activity
Callistephus chinensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.171
glutaryl-CoA + pelargonidin 3-O-beta-D-glucoside
50% of the activity with malonyl-CoA
656933
Callistephus chinensis
CoA + pelargonidin 3-O-(6-O-glutaryl-beta-D-glucoside)
-
-
-
?
2.3.1.171
malonyl-CoA + cyanidin 3-O-beta-D-glucoside
-
656933
Callistephus chinensis
CoA + cyanidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
-
-
?
2.3.1.171
malonyl-CoA + delphinidin 3,5-di-O-beta-D-glucoside
10% of the activity with pelargonidin 3-O-glucoside
656933
Callistephus chinensis
CoA + ?
-
-
-
?
2.3.1.171
malonyl-CoA + delphinidin 3-O-beta-D-glucoside
-
656933
Callistephus chinensis
CoA + delphinidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
-
-
?
2.3.1.171
malonyl-CoA + pelargonidin 3-O-beta-D-glucoside
-
656933
Callistephus chinensis
CoA + pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
-
-
?
2.3.1.171
methylmalonyl-CoA + pelargonidin 3-O-beta-D-glucoside
30% of the activity with malonyl-CoA
656933
Callistephus chinensis
CoA + pelargonidin 3-O-(6-O-methylmalonyl-beta-D-glucoside)
-
-
-
?
2.3.1.171
succinyl-CoA + pelargonidin 3-O-beta-D-glucoside
40% of the activity with malonyl-CoA
656933
Callistephus chinensis
CoA + pelargonidin 3-O-(6-O-succinyl-beta-D-glucoside)
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.171
50
-
-
Callistephus chinensis
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.1.171
30
80
50% of maximal activity at 30°C and at 80°C, 0°C: 17% of maximal activity activity
Callistephus chinensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.171
7
-
-
Callistephus chinensis