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Literature summary extracted from

  • Motyka, V.; Vankova, R.; Capkova, V.; Petrasek, J.; Kaminek, M.; Schmulling, T.
    Cytokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion (2003), Physiol. Plant., 117, 11-21.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.99.12 additional information cytokinin-induced up-regulation of enzyme activity, e.g. by N6-benzylaminopurine application on the plant leaf or callus surface leads to about 2.5fold increased activity after 4 h, overview Nicotiana tabacum

Protein Variants

EC Number Protein Variants Comment Organism
1.5.99.12 additional information construction of transgenic tobacco plants conditionally overexpressing isopentenyltransferase IPT, tetracycline-induced derepressing of the IPT transcription, results in enhanced cytokinin oxidase activity Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.12 additional information
-
 additional information enzyme activity dynamics in relation to glycosylation level Nicotiana tabacum
1.5.99.12 0.001
-
 N6-(DELTA2-isopentenyl)adenine transgenic plant cells conditionally overexpressing isopentenyltransferase, cell culture medium induced by tetracycline, pH 6.0, 37°C Nicotiana tabacum
1.5.99.12 0.0015
-
 N6-(DELTA2-isopentenyl)adenine transgenic plant cells conditionally overexpressing isopentenyltransferase, cell extract induced by tetracycline, pH 6.0, 37°C Nicotiana tabacum
1.5.99.12 0.0017
-
 N6-(DELTA2-isopentenyl)adenine transgenic plant cells conditionally overexpressing isopentenyltransferase, cell culture medium induced by N6-benzylaminopurine, pH 6.0, 37°C Nicotiana tabacum
1.5.99.12 0.0022
-
 N6-(DELTA2-isopentenyl)adenine transgenic plant cells conditionally overexpressing isopentenyltransferase, cell extract, pH 6.0, 37°C Nicotiana tabacum
1.5.99.12 0.0023
-
 N6-(DELTA2-isopentenyl)adenine transgenic plant cells conditionally overexpressing isopentenyltransferase, cell extract induced by N6-benzylaminopurine, pH 6.0, 37°C Nicotiana tabacum
1.5.99.12 0.0025
-
 N6-(DELTA2-isopentenyl)adenine transgenic plant cells conditionally overexpressing isopentenyltransferase, cell culture medium, pH 6.0, 37°C Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.12 additional information Nicotiana tabacum endogenous cytokinin levels after induction with N6-benzylaminopurine, overview, apoplastic enzyme degradation pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.12 Nicotiana tabacum
-
 cv. Wisconsin 38, wild-type, and transgenic tobacco conditionally overexpressing isopentenyltransferase IPT
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.5.99.12 glycoprotein enzyme binds with moderate affinity to concanavalin A, glycosylation is influenced by cytokinins and has a regulatory role for the enzyme activity, glycosylation changes the enzyme secretion level Nicotiana tabacum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.12 partial from leaves and calli of wild-type and transgenic plants Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.99.12 callus
-
 Nicotiana tabacum
-
1.5.99.12 cell suspension culture
-
 Nicotiana tabacum
-
1.5.99.12 leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.12 0.0003
-
 transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium, not induced Nicotiana tabacum
1.5.99.12 0.0003
-
 wild-type plant, cell extract and cell culture medium, not induced or tetracycline-treated cells Nicotiana tabacum
1.5.99.12 0.0004
-
 transgenic plant conditionally overexpressing isopentenyltransferase, cell extract, not induced cells Nicotiana tabacum
1.5.99.12 0.002
-
 wild-type plant and transgenic plant conditionally overexpressing isopentenyltransferase, cell extract, N6-benzylaminopurine-induced Nicotiana tabacum
1.5.99.12 0.004
-
 transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium of N6-benzylaminopurine-induced cells Nicotiana tabacum
1.5.99.12 0.005
-
 wild-type plant, cell culture medium of N6-benzylaminopurine-induced cells Nicotiana tabacum
1.5.99.12 0.0065
-
 transgenic plant conditionally overexpressing isopentenyltransferase, cell culture medium of tetracycline-treated cells Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.12 additional information endogenous cytokinin levels after induction with N6-benzylaminopurine, overview, apoplastic enzyme degradation pathway Nicotiana tabacum ?
-
 ?
1.5.99.12 N6-(DELTA2-isopentenyl)adenine + acceptor + H2O
-
 Nicotiana tabacum adenine + 3-methylbut-2-enal + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.12 CKX
-
 Nicotiana tabacum
1.5.99.12 cytokinin oxidase
-
 Nicotiana tabacum
1.5.99.12 More see also EC 1.4.3.18 Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.12 6
-
 secreted enzyme in cell culture medium Nicotiana tabacum
1.5.99.12 8.5
-
 enzyme in cells Nicotiana tabacum