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Literature summary extracted from
- Purification and characterization of an endoamylase from tulip (Tulipa gesneriana) bulbs (2000), Physiol. Plant., 109, 388-395.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | dithiothreitol | 10 mM, slight activation | Tulipa gesneriana |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | 5,5'-dithiobis-[2-nitrobenzoic acid] | 10 mM, 35% inhibition | Tulipa gesneriana | |
| 3.2.1.1 | AgNO3 | 2 mM, complete inhibition | Tulipa gesneriana | |
| 3.2.1.1 | CuSO4 | 2 mM, 33% inhibition | Tulipa gesneriana | |
| 3.2.1.1 | EDTA | 2 mM, 25% inhibition | Tulipa gesneriana | |
| 3.2.1.1 | HgCl2 | 2 mM, complete inhibition | Tulipa gesneriana | |
| 3.2.1.1 | N-ethylmaleimide | 10 mM, 22% inhibition | Tulipa gesneriana | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
starch | 1.28 mg/ml, 30°C, pH 6.0 | Tulipa gesneriana | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 45000 | - |
gel filtration | Tulipa gesneriana |
| 3.2.1.1 | 51000 | - |
1 * 51000, SDS-PAGE | Tulipa gesneriana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | starch + H2O | Tulipa gesneriana | - |
fragments of starch | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Tulipa gesneriana | - |
L. cv. Apeldoorn | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | ammonium sulfate, DEAE-Sephacel, Phenyl-agarose, Sephacryl S-200, chromatofocusing | Tulipa gesneriana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | bulb | - |
Tulipa gesneriana | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 464 | - |
- |
Tulipa gesneriana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | amylopectin + H2O | - |
Tulipa gesneriana | fragments of amylopectin | - |
? | |
| 3.2.1.1 | amylose + H2O | - |
Tulipa gesneriana | fragments of amylose | - |
? | |
| 3.2.1.1 | beta-limit dextrin + H2O | - |
Tulipa gesneriana | fragments of beta-limit dextrin | - |
? | |
| 3.2.1.1 | glycogen + H2O | 29% of activity with starch | Tulipa gesneriana | fragments of glycogen | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Tulipa gesneriana | fragments of starch | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | monomer | 1 * 51000, SDS-PAGE | Tulipa gesneriana |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | amylase I | - |
Tulipa gesneriana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 55 | - |
- |
Tulipa gesneriana |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 20 | 70 | approx. 20% of maximal activity at 20°C, approx. 40% of maximal activity at 65°C | Tulipa gesneriana |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 20 | - |
approx. 20% loss of activity after 30 min, approx. 10% loss of activity in the presence of 5 mM CaCl2 | Tulipa gesneriana |
| 3.2.1.1 | 30 | - |
approx. 30% loss of activity after 30 min, approx. 15% loss of activity in the presence of 5 mM CaCl2 | Tulipa gesneriana |
| 3.2.1.1 | 40 | - |
approx. 50% loss of activity after 30 min, approx. 25% loss of activity in the presence of 5 mM CaCl2 | Tulipa gesneriana |
| 3.2.1.1 | 50 | - |
almost complete loss of activity after 30 min, approx. 30% loss of activity in the presence of 5 mM CaCl2 | Tulipa gesneriana |
| 3.2.1.1 | 60 | - |
approx. 65% loss of activity in the presence of 5 mM CaCl2 | Tulipa gesneriana |
| 3.2.1.1 | 70 | - |
almost complete loss of activity in the presence of 5 mM CaCl2 | Tulipa gesneriana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 6 | - |
- |
Tulipa gesneriana |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 3.5 | 9 | approx. 30% of maximal activity at pH 3.5, approx. 20% of maximal activity at pH 9.0 | Tulipa gesneriana |
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