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Literature summary extracted from

  • Calin-Jageman, I.; Amarasinghe, A.K.; Nicholson, A.W.
    Ethidium-dependent uncoupling of substrate binding and cleavage by Escherichia coli ribonuclease III (2001), Nucleic Acids Res., 29, 1915-1925.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.3 E117Q mutant enzyme can still bind to the substrate RNA in presence of Mg2+ but cannot cleave it Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.3 Ethidium bromide reversible, competitive inhibition in vitro, uncouples small RNA substrate binding and cleavage, intercalation inhibition mechanism, perturbation of substrate recognition by the N-terminal catalytic domain, 81% inhibition at 0.02 mM, tRNA abolishes the inhibitory effect Escherichia coli
3.1.26.3 tRNA inhibits enzyme activity and inhibition by ethidium bromide Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.3 additional information
-
 additional information substrate binding kinetics, wild-type and mutant enzyme, in presence or absence of ethidium bromide Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.3 Mg2+ required Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.3 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.3 ds-rRNA + H2O double-strand RNA specific endonuclease Escherichia coli mature ds-rRNA
-
 ?
3.1.26.3 R1.1 RNA + H2O based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, substrate possesses 2 ethidium bromide binding sites in the internal loop and the lower stem, respectively, both consisting of an A-A pair stacked on a CG pair, which is a motif that is a favourable environment for intercalation Escherichia coli fragments of R1.1 RNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.3 RNase III
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.3 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.3 8
-
 assay at Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.26.3 0.0017
-
 Ethidium bromide pH 8.0, 37°C Escherichia coli