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Literature summary extracted from
- Identification and characterization of an extracellular acid trehalase from the ectomycorrhizal fungus Amanita muscaria (2000), New Phytol., 146, 169-175.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | sucrose | competitive | Amanita muscaria |  |
| 3.2.1.28 | validamycin | competitive | Amanita muscaria | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | 0.38 | - |
alpha,alpha-trehalose | - |
Amanita muscaria | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.28 | cell wall | - |
Amanita muscaria | 5618 | - |
| 3.2.1.28 | extracellular | - |
Amanita muscaria | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 135000 | - |
x * 135000, SDS-PAGE | Amanita muscaria |
| 3.2.1.28 | 165000 | - |
gel filtration | Amanita muscaria |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Amanita muscaria | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | - |
Amanita muscaria |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | culture medium | - |
Amanita muscaria | - |
| 3.2.1.28 | mycelium | - |
Amanita muscaria | - |
| 3.2.1.28 | mycorrhiza | activity is strongly decreased in ectomycorrhizas | Amanita muscaria | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O | highly specific for | Amanita muscaria | alpha-D-glucopyranose + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | ? | x * 135000, SDS-PAGE | Amanita muscaria |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | 0.000045 | - |
validamycin | - |
Amanita muscaria | |
| 3.2.1.28 | 15 | - |
sucrose | - |
Amanita muscaria | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.28 | Amanita muscaria | - |
- |
4.2 |
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