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Literature summary extracted from

  • Khunsook, S.; Alhadeff, J.A.; Bean, B.S.
    Purification and characterization of human seminal plasma alpha-L-fucosidase (2002), Mol. Hum. Reprod., 8, 221-227.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.06
-
 4-methylumbelliferyl alpha-L-fucopyranoside pH 7.0, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 236000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 sialoglycoprotein the enzyme occurs in different isoforms depending on the number of terminal sialic acid residues present on the glycoprotein molecules Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 liver
-
 Homo sapiens
-
3.2.1.51 seminal plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 83
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 -20°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 6 weeks Homo sapiens
3.2.1.51 2-4°C, pH 5.5 in presence of 1% L-fucose, 77% activity after 6 weeks Homo sapiens
3.2.1.51 21°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 3 days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-methylumbelliferyl alpha-L-fucopyranoside
-
 Homo sapiens 4-methylumbelliferone + alpha-L-fucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 tetramer 4 * 56000-57000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 37
-
 100% activity after 6 h Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 4 4.5 a broad acidic optimum at pH 4.0-4.5 and a second neutral optimum at pH 6.4-7.4 Homo sapiens
3.2.1.51 6.4 7.4 a broad acidic optimum at pH 4.0-4.5 and a second neutral optimum at pH 6.4-7.4 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.51 Homo sapiens isoelectric focusing indicates that the purified enzyme consists of multiple isoforms
-
 additional information