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Literature summary extracted from

  • Guescini, M.; Pierleoni, R.; Palma, F.; Zeppa, S.; Vallorani, L.; Potenza, L.; Sacconi, C.; Giomaro, G.; Stocchi, V.
    Characterization of the Tuber borchii nitrate reductase gene and its role in ectomycorrhizae (2003), Mol. Genet. Genomics, 269, 807-816.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.3 additional information enzyme is nitrate-inducible Tuber borchii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.3 gene tbnr1, DNA and amino acid sequence determination and analysis, expression analysis Tuber borchii
1.7.1.3 gene tpnr1, DNA sequence determination and analysis, construction of a cDNA library Tilia platyphyllos

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.3 nitrate + NADPH Tilia platyphyllos enzyme is involved in nitrate assimilation nitrite + NADP+
-
 ?
1.7.1.3 nitrate + NADPH Tuber borchii enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis nitrite + NADP+
-
 ?
1.7.1.3 nitrate + NADPH Tuber borchii Z43 enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis nitrite + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Tilia platyphyllos Q8H1T7 gene tpnr1
-
1.7.1.3 Tuber borchii Q8J259 gene tbnr1, ascomycete
-
1.7.1.3 Tuber borchii Z43 Q8J259 gene tbnr1, ascomycete
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.3 ectomycorrhiza symbiosis with Tilia platyphyllos, root Tuber borchii
-
1.7.1.3 ectomycorrhiza symbiosis with Tuber borchii, mycelium Tilia platyphyllos
-
1.7.1.3 additional information the enzymatic activity is increased in root ectomycchorizae in symbiosis with the fungus Tuber borchii, while the expression of the endogenous enzyme is decreased Tilia platyphyllos
-
1.7.1.3 additional information the enzyme expression and activity is increased in ectomycchorizae in symbiosis with Tilia platyphyllos Tuber borchii
-
1.7.1.3 mycelium
-
 Tuber borchii
-
1.7.1.3 root non-inoculated Tilia platyphyllos
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.3 0.0014
-
 root Tilia platyphyllos
1.7.1.3 0.003
-
 ectomycchorizae Tilia platyphyllos

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + NADPH
-
 Tuber borchii nitrite + NADP+
-
 ?
1.7.1.3 nitrate + NADPH
-
 Tilia platyphyllos nitrite + NADP+
-
 ?
1.7.1.3 nitrate + NADPH enzyme is involved in nitrate assimilation Tilia platyphyllos nitrite + NADP+
-
 ?
1.7.1.3 nitrate + NADPH enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis Tuber borchii nitrite + NADP+
-
 ?
1.7.1.3 nitrate + NADPH
-
 Tuber borchii Z43 nitrite + NADP+
-
 ?
1.7.1.3 nitrate + NADPH enzyme is involved in nitrate assimilation, it serves the nitrogen nutrition of the host plant of Tuber borchii in symbiosis Tuber borchii Z43 nitrite + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.3 nitrate reductase
-
 Tuber borchii
1.7.1.3 nitrate reductase
-
 Tilia platyphyllos

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.3 7.4
-
 assay at Tuber borchii
1.7.1.3 7.4
-
 assay at Tilia platyphyllos

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.3 additional information enzyme amino acid sequence contains a molybdate-cofactor, a cytochrome b5 heme, and a FAD binding domain Tuber borchii
1.7.1.3 NADPH dependent on Tuber borchii
1.7.1.3 NADPH dependent on Tilia platyphyllos