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Literature summary extracted from

  • Thomas, J.L.; Duax, W.L.; Addlagatta, A.; Kacsoh, B.; Brandt, S.E.; Norris, W.B.
    Structure/function aspects of human 3beta-hydroxysteroid dehydrogenase (2004), Mol. Cell. Endocrinol., 215, 73-82.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 expression in Sf9 cells Homo sapiens
1.1.1.145 expression in Sf9 insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.145 D241N 39% of wild-type activity Homo sapiens
1.1.1.145 D257L trace activity Homo sapiens
1.1.1.145 D258L trace activity Homo sapiens
1.1.1.145 D265N 12% of wild-type activity Homo sapiens
1.1.1.145 D36A/K37R uses NADP+ as cofactor, no activity with NAD+ Homo sapiens
1.1.1.145 H156Y 195% of wild-type activity Homo sapiens
1.1.1.145 K158Q no activity Homo sapiens
1.1.1.145 K273Q no activity Homo sapiens
1.1.1.145 Y154F no activity Homo sapiens
1.1.1.145 Y269S no activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 epostane
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.0037
-
 dehydroepiandrosterone 27°C, pH 7.4 Homo sapiens
1.1.1.145 0.025
-
 NAD+ 27°C, pH 7.4, H156Y mutant Homo sapiens
1.1.1.145 0.034
-
 NAD+ 27°C, pH 7.4 Homo sapiens
1.1.1.145 0.042
-
 dehydroepiandrosterone 27°C, pH 7.4, H156Y mutant Homo sapiens
1.1.1.145 0.047
-
 dehydroepiandrosterone 27°C, pH 7.4 Homo sapiens
1.1.1.145 0.086
-
 NAD+ 27°C, pH 7.4 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.145 42000
-
 x * 42000, SDS-PAGE, recombinant 3beta-HSD Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+ Homo sapiens
-
 androst-5-en-3,17-dione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.145 recombinant 3beta-HSD Homo sapiens
1.1.1.145 recombinant 3beta-HSD type 2 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 breast cancer cell
-
 Homo sapiens
-
1.1.1.145 gonad
-
 Homo sapiens
-
1.1.1.145 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Homo sapiens androst-5-en-3,17-dione + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.145 ? x * 42000, SDS-PAGE, recombinant 3beta-HSD Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 3beta-hydroxysteroid dehydrogenase type 1
-
 Homo sapiens
1.1.1.145 3beta-hydroxysteroid dehydrogenase type 2
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.145 0.00007
-
 epostane 27°C, pH 7.4 Homo sapiens
1.1.1.145 0.00098
-
 epostane 27°C, pH 7.4 Homo sapiens