BRENDA - Enzyme Database

A comparative study of the isocitrate dehydrogenases of Chlorobium limicola forma thiosulfatophilum and Rhodopseudomonas palustris

Lebedeva, N.V.; Malinina, N.V.; Ivanovsky, R.N.; Microbiology 71, 657-662 (2002)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
50% inhibition of the forward reaction at 1.5 mM, 99% inhibition at 15 mM
Chlorobium limicola
1.1.1.42
2-oxoglutarate
50% inhibition of the forward reaction at 1.5 mM, 95% inhibition at 15 mM
Rhodopseudomonas palustris
1.1.1.42
isocitrate
50% inhibition of the reverse reaction at 0.1 mM, 97% inhibition at 1.2 mM
Chlorobium limicola
1.1.1.42
isocitrate
70% inhibition of the reverse reaction at 0.1 mM, 99% inhibition at 1.2 mM
Rhodopseudomonas palustris
1.1.1.42
additional information
no inhibition by glyoxylate
Chlorobium limicola
1.1.1.42
additional information
no inhibition by glyoxylate
Rhodopseudomonas palustris
1.1.1.42
NADP+
37% inhibition of the reverse reaction at 4.0 mM
Chlorobium limicola
1.1.1.42
NADP+
35% inhibition of the reverse reaction at 4.0 mM
Rhodopseudomonas palustris
1.1.1.42
NADPH
42% inhibition of the forward reaction at 0.5 mM
Chlorobium limicola
1.1.1.42
NADPH
66% inhibition of the forward reaction at 0.5 mM
Rhodopseudomonas palustris
1.1.1.42
NaHCO3
28% inhibition of the forward reaction at 10 mM
Chlorobium limicola
1.1.1.42
NaHCO3
9% inhibition of the forward reaction at 10 mM
Rhodopseudomonas palustris
1.1.1.42
oxaloacetate
18.6% inhibition at 2.5 mM
Chlorobium limicola
1.1.1.42
oxaloacetate
15.5% inhibition at 2.5 mM
Rhodopseudomonas palustris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.001
-
isocitrate
pH 8.0, forward reaction
Chlorobium limicola
1.1.1.42
0.007
-
NADP+
pH 8.0, forward reaction
Chlorobium limicola
1.1.1.42
0.009
-
isocitrate
pH 8.0, forward reaction
Rhodopseudomonas palustris
1.1.1.42
0.01
-
NADPH
pH 6.5, reverse reaction
Chlorobium limicola
1.1.1.42
0.017
-
NADP+
pH 8.0, forward reaction
Rhodopseudomonas palustris
1.1.1.42
0.034
-
NADPH
pH 6.5, reverse reaction
Rhodopseudomonas palustris
1.1.1.42
0.304
-
2-oxoglutarate
pH 6.5, reverse reaction
Chlorobium limicola
1.1.1.42
0.398
-
2-oxoglutarate
pH 6.5, reverse reaction
Rhodopseudomonas palustris
1.1.1.42
11.68
-
NaHCO3
pH 6.5, reverse reaction
Chlorobium limicola
1.1.1.42
13.82
-
NaHCO3
pH 6.5, reverse reaction
Rhodopseudomonas palustris
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Chlorobium limicola
5829
-
1.1.1.42
cytosol
-
Rhodopseudomonas palustris
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
divalent cation is required, optimal activity at 40 mM in the forward reaction, and at 250 mM in the reverse reaction
Chlorobium limicola
1.1.1.42
Mg2+
divalent cation is required, optimal activity at 2 mM in the forward reaction, and at 200 mM in the reverse reaction
Rhodopseudomonas palustris
1.1.1.42
Mn2+
divalent cation is required, optimal activity at 10 mM in the forward reaction, and at 50 mM in the reverse reaction
Chlorobium limicola
1.1.1.42
Mn2+
divalent cation is required, optimal activity at 1 mM in the forward reaction, and at 20 mM in the reverse reaction
Rhodopseudomonas palustris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
64400
-
gel filtration
Chlorobium limicola
1.1.1.42
69100
-
gel filtration
Rhodopseudomonas palustris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Chlorobium limicola
metabolic regulation, involved in the tricarboxylic acid cycle
2-oxoglutarate + CO2 + NADPH
-
-
r
1.1.1.42
isocitrate + NADP+
Rhodopseudomonas palustris
metabolic regulation, involved in the tricarboxylic acid cycle
2-oxoglutarate + CO2 + NADPH
-
-
r
1.1.1.42
isocitrate + NADP+
Rhodopseudomonas palustris 285
metabolic regulation, involved in the tricarboxylic acid cycle
2-oxoglutarate + CO2 + NADPH
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Chlorobium limicola
-
formerly Chlorobium thiosulfatophilum, strain L
-
1.1.1.42
Rhodopseudomonas palustris
-
-
-
1.1.1.42
Rhodopseudomonas palustris 285
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
41fold by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting
Chlorobium limicola
1.1.1.42
by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting
Rhodopseudomonas palustris
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.42
0.04
-
purified enzyme, reverse reaction
Chlorobium limicola
1.1.1.42
0.28
-
purified enzyme, forward reaction
Chlorobium limicola
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.42
-18░C, purified enzyme, 50% glycerol, stable for at least 1.5-2 months
Chlorobium limicola
1.1.1.42
-18░C, purified enzyme, 50% glycerol, stable for at least 1.5-2 months
Rhodopseudomonas palustris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
656740
Chlorobium limicola
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
656740
Rhodopseudomonas palustris
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
metabolic regulation, involved in the tricarboxylic acid cycle
656740
Chlorobium limicola
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
metabolic regulation, involved in the tricarboxylic acid cycle
656740
Rhodopseudomonas palustris
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
656740
Rhodopseudomonas palustris 285
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
metabolic regulation, involved in the tricarboxylic acid cycle
656740
Rhodopseudomonas palustris 285
2-oxoglutarate + CO2 + NADPH
-
-
-
r
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
1.1.1.42
50
-
5 min, complete loss of activity
Chlorobium limicola
1.1.1.42
50
-
5 min, complete loss of activity
Rhodopseudomonas palustris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
6.1
-
reverse reaction
Rhodopseudomonas palustris
1.1.1.42
6.7
-
reverse reaction
Chlorobium limicola
1.1.1.42
8.1
-
forward reaction
Chlorobium limicola
1.1.1.42
8.3
-
forward reaction
Rhodopseudomonas palustris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Chlorobium limicola
1.1.1.42
NADP+
-
Rhodopseudomonas palustris
1.1.1.42
NADPH
-
Chlorobium limicola
1.1.1.42
NADPH
-
Rhodopseudomonas palustris
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.1.42
Rhodopseudomonas palustris
isoelectric focusing
-
4.9
1.1.1.42
Chlorobium limicola
isoelectric focusing
-
6
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Chlorobium limicola
1.1.1.42
NADP+
-
Rhodopseudomonas palustris
1.1.1.42
NADPH
-
Chlorobium limicola
1.1.1.42
NADPH
-
Rhodopseudomonas palustris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
50% inhibition of the forward reaction at 1.5 mM, 99% inhibition at 15 mM
Chlorobium limicola
1.1.1.42
2-oxoglutarate
50% inhibition of the forward reaction at 1.5 mM, 95% inhibition at 15 mM
Rhodopseudomonas palustris
1.1.1.42
isocitrate
50% inhibition of the reverse reaction at 0.1 mM, 97% inhibition at 1.2 mM
Chlorobium limicola
1.1.1.42
isocitrate
70% inhibition of the reverse reaction at 0.1 mM, 99% inhibition at 1.2 mM
Rhodopseudomonas palustris
1.1.1.42
additional information
no inhibition by glyoxylate
Chlorobium limicola
1.1.1.42
additional information
no inhibition by glyoxylate
Rhodopseudomonas palustris
1.1.1.42
NADP+
37% inhibition of the reverse reaction at 4.0 mM
Chlorobium limicola
1.1.1.42
NADP+
35% inhibition of the reverse reaction at 4.0 mM
Rhodopseudomonas palustris
1.1.1.42
NADPH
42% inhibition of the forward reaction at 0.5 mM
Chlorobium limicola
1.1.1.42
NADPH
66% inhibition of the forward reaction at 0.5 mM
Rhodopseudomonas palustris
1.1.1.42
NaHCO3
28% inhibition of the forward reaction at 10 mM
Chlorobium limicola
1.1.1.42
NaHCO3
9% inhibition of the forward reaction at 10 mM
Rhodopseudomonas palustris
1.1.1.42
oxaloacetate
18.6% inhibition at 2.5 mM
Chlorobium limicola
1.1.1.42
oxaloacetate
15.5% inhibition at 2.5 mM
Rhodopseudomonas palustris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.001
-
isocitrate
pH 8.0, forward reaction
Chlorobium limicola
1.1.1.42
0.007
-
NADP+
pH 8.0, forward reaction
Chlorobium limicola
1.1.1.42
0.009
-
isocitrate
pH 8.0, forward reaction
Rhodopseudomonas palustris
1.1.1.42
0.01
-
NADPH
pH 6.5, reverse reaction
Chlorobium limicola
1.1.1.42
0.017
-
NADP+
pH 8.0, forward reaction
Rhodopseudomonas palustris
1.1.1.42
0.034
-
NADPH
pH 6.5, reverse reaction
Rhodopseudomonas palustris
1.1.1.42
0.304
-
2-oxoglutarate
pH 6.5, reverse reaction
Chlorobium limicola
1.1.1.42
0.398
-
2-oxoglutarate
pH 6.5, reverse reaction
Rhodopseudomonas palustris
1.1.1.42
11.68
-
NaHCO3
pH 6.5, reverse reaction
Chlorobium limicola
1.1.1.42
13.82
-
NaHCO3
pH 6.5, reverse reaction
Rhodopseudomonas palustris
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Chlorobium limicola
5829
-
1.1.1.42
cytosol
-
Rhodopseudomonas palustris
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
divalent cation is required, optimal activity at 40 mM in the forward reaction, and at 250 mM in the reverse reaction
Chlorobium limicola
1.1.1.42
Mg2+
divalent cation is required, optimal activity at 2 mM in the forward reaction, and at 200 mM in the reverse reaction
Rhodopseudomonas palustris
1.1.1.42
Mn2+
divalent cation is required, optimal activity at 10 mM in the forward reaction, and at 50 mM in the reverse reaction
Chlorobium limicola
1.1.1.42
Mn2+
divalent cation is required, optimal activity at 1 mM in the forward reaction, and at 20 mM in the reverse reaction
Rhodopseudomonas palustris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
64400
-
gel filtration
Chlorobium limicola
1.1.1.42
69100
-
gel filtration
Rhodopseudomonas palustris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Chlorobium limicola
metabolic regulation, involved in the tricarboxylic acid cycle
2-oxoglutarate + CO2 + NADPH
-
-
r
1.1.1.42
isocitrate + NADP+
Rhodopseudomonas palustris
metabolic regulation, involved in the tricarboxylic acid cycle
2-oxoglutarate + CO2 + NADPH
-
-
r
1.1.1.42
isocitrate + NADP+
Rhodopseudomonas palustris 285
metabolic regulation, involved in the tricarboxylic acid cycle
2-oxoglutarate + CO2 + NADPH
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
41fold by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting
Chlorobium limicola
1.1.1.42
by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting
Rhodopseudomonas palustris
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.42
0.04
-
purified enzyme, reverse reaction
Chlorobium limicola
1.1.1.42
0.28
-
purified enzyme, forward reaction
Chlorobium limicola
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.42
-18░C, purified enzyme, 50% glycerol, stable for at least 1.5-2 months
Chlorobium limicola
1.1.1.42
-18░C, purified enzyme, 50% glycerol, stable for at least 1.5-2 months
Rhodopseudomonas palustris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
656740
Chlorobium limicola
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
656740
Rhodopseudomonas palustris
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
metabolic regulation, involved in the tricarboxylic acid cycle
656740
Chlorobium limicola
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
metabolic regulation, involved in the tricarboxylic acid cycle
656740
Rhodopseudomonas palustris
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
656740
Rhodopseudomonas palustris 285
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
metabolic regulation, involved in the tricarboxylic acid cycle
656740
Rhodopseudomonas palustris 285
2-oxoglutarate + CO2 + NADPH
-
-
-
r
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
1.1.1.42
50
-
5 min, complete loss of activity
Chlorobium limicola
1.1.1.42
50
-
5 min, complete loss of activity
Rhodopseudomonas palustris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
6.1
-
reverse reaction
Rhodopseudomonas palustris
1.1.1.42
6.7
-
reverse reaction
Chlorobium limicola
1.1.1.42
8.1
-
forward reaction
Chlorobium limicola
1.1.1.42
8.3
-
forward reaction
Rhodopseudomonas palustris
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.1.42
Rhodopseudomonas palustris
isoelectric focusing
-
4.9
1.1.1.42
Chlorobium limicola
isoelectric focusing
-
6