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Literature summary extracted from

  • Li, C.; Florova, G.; Akopiants, K.; Reynolds, K.A.
    Crotonyl-coenzyme A reductase provides methylmalonyl-CoA precursors for monensin biosynthesis by Streptomyces cinnamonensis in an oil-based extended fermentation (2004), Microbiology, 150, 3463-3472.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.8 biotechnology optimization of oil-based extended fermentation of recombinant Streptomyces cinnamonensis, expressing the enzyme from Streptomyces collinus, is used to provide methylmalonyl-CoA precursors for monensin biosynthesis, overview Streptomyces collinus
1.3.1.8 synthesis oil-based extended fermentation of recombinant Streptomyces cinnamonensis, expressing the enzyme from Streptomyces collinus, is used to provide methylmalonyl-CoA precursors for monensin biosynthesis Streptomyces collinus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.8 expression in Streptomyces cinnamonensis strains C730.1 and L1 Streptomyces collinus
1.3.1.86 expressed in Streptomyces cinnamonensis strain L1 Streptomyces collinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.8 crotonyl-CoA + reduced acceptor Streptomyces collinus enzyme is involved in synthesizing methylmalonyl-CoA precursors for monensin biosynthesis, pathway flux, overview butyryl-CoA + oxidized acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.8 Streptomyces collinus
-
 gene ccr, enzyme CCR
-
1.3.1.86 Streptomyces collinus
-
-
-
1.3.1.86 Streptomyces virginiae
-
 strain C730.1
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.8 acyl-CoA + NADP+ = 2,3-dehydroacyl-CoA + NADPH + H+ enzyme might also belong to EC 1.3.99.3, acyl-CoA dehydrogenase Streptomyces collinus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.8 additional information fermentation in an oil-based medium Streptomyces collinus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.8 additional information
-
 quantification by determination of monensin A Streptomyces collinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.8 crotonyl-CoA + reduced acceptor
-
 Streptomyces collinus butyryl-CoA + oxidized acceptor
-
 ?
1.3.1.8 crotonyl-CoA + reduced acceptor enzyme is involved in synthesizing methylmalonyl-CoA precursors for monensin biosynthesis, pathway flux, overview Streptomyces collinus butyryl-CoA + oxidized acceptor
-
 ?
1.3.1.86 crotonyl-CoA + NADPH + H+
-
 Streptomyces virginiae butyryl-CoA + NADP+
-
 ?
1.3.1.86 crotonyl-CoA + NADPH + H+
-
 Streptomyces collinus butyryl-CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.8 CCR ambiguous Streptomyces collinus
1.3.1.8 crotonyl-coenzyme A reductase
-
 Streptomyces collinus
1.3.1.86 CCR
-
 Streptomyces virginiae
1.3.1.86 CCR
-
 Streptomyces collinus
1.3.1.86 crotonyl-coenzyme A reductase
-
 Streptomyces virginiae
1.3.1.86 crotonyl-coenzyme A reductase
-
 Streptomyces collinus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.86 NADPH
-
 Streptomyces virginiae
1.3.1.86 NADPH
-
 Streptomyces collinus

General Information

EC Number General Information Comment Organism
1.3.1.86 malfunction loss of ccr leads to lower levels of the monensin precursor methymalonyl-CoA, relative to coenzyme A Streptomyces virginiae
1.3.1.86 malfunction loss of ccr leads to lower levels of the monensin precursor methymalonyl-CoA, relative to coenzyme A Streptomyces collinus
1.3.1.86 physiological function CCR plays a significant role in providing methylmalonyl-CoA for monensin biosynthesis in oil-based 10-day fermentations of Streptomyces cinnamonensis Streptomyces virginiae
1.3.1.86 physiological function CCR plays a significant role in providing methylmalonyl-CoA for monensin biosynthesis in oil-based 10-day fermentations of Streptomyces cinnamonensis Streptomyces collinus