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Literature summary extracted from

  • Kiiskinen, L.L.; Kruus, K.; Bailey, M.; Ylosmaki, E.; Siika-Aho, M.; Saloheimo, M.
    Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme (2004), Microbiology, 150, 3065-3074.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 biotechnology expression of non-fused enzyme and hydrophobin-enzyme fusion protein in Trichoderma reesei, intracellular accumulation and degradation of fusion protein, production of non-fused enzyme at up to 920 mg per l of fed-batch culture, purification from culture supernatant Melanocarpus albomyces

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0018
-
 syringaldazine pH 6.0 Melanocarpus albomyces
1.10.3.2 0.0052
-
 2,6-dimethoxyphenol pH 6.0 Melanocarpus albomyces
1.10.3.2 0.27
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5 Melanocarpus albomyces
1.10.3.2 0.91
-
 guaiacol pH 6.0 Melanocarpus albomyces

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Melanocarpus albomyces
-
 expression inTrichoderma reesei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 150
-
 substrate guaiacol, pH 6.0 Melanocarpus albomyces
1.10.3.2 400
-
 substrate 2,6-dimethoxypenol, pH 6.0 Melanocarpus albomyces
1.10.3.2 850
-
 substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 4.5 Melanocarpus albomyces
1.10.3.2 1070
-
 substrate syringaldazine, pH 6.0 Melanocarpus albomyces

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Melanocarpus albomyces ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Melanocarpus albomyces ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Melanocarpus albomyces ? + H2O
-
 ?
1.10.3.2 syringaldazine + O2
-
 Melanocarpus albomyces ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 31.7
-
 guaiacol pH 6.0 Melanocarpus albomyces
1.10.3.2 67
-
 2,6-dimethoxyphenol pH 6.0 Melanocarpus albomyces
1.10.3.2 78.3
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5 Melanocarpus albomyces
1.10.3.2 92
-
 syringaldazine pH 6.0 Melanocarpus albomyces