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Literature summary extracted from

  • Baughn, A.D.; Malamy, M.H.
    The essential role of fumarate reductase in haem-dependent growth stimulation of Bacteroides fragilis (2003), Microbiology, 149, 1551-1558.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.6 additional information heme induces enzyme expression and is required for optimal anaerobic growth Bacteroides fragilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.6 genes frdA, frdB, and frdC, organized in an operon, DNA sequence determination and analysis, subcloning in Escherichia coli, expression of mutant enzymes Bacteroides fragilis

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.6 additional information construction of in frame-deletions mutants of frdC and frdB, mutation results in mutants with no catalytic activity and threefold increased generation time compared to the wild-type strain, growth can partially be restored by addition of succinate to the medium Bacteroides fragilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.6 Fe2+ heme cofactor Bacteroides fragilis
1.3.1.6 Mg2+
-
 Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.6 fumarate + NADH + H+ Bacteroides fragilis enzyme is required for optimal growth in presence of heme, enzyme plays a role in the generation of metabolic energy succinate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.6 Bacteroides fragilis Q7X478 iron-sulfur cluster protein subunit
-
1.3.1.6 Bacteroides fragilis Q7X479 flavoprotein subunit
-
1.3.1.6 Bacteroides fragilis Q7X480 cytochrome b subunit
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.6 26
-
 wild-type strain, assay under anaerobic conditions Bacteroides fragilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.6 fumarate + NADH + H+
-
 Bacteroides fragilis succinate + NAD+
-
 ?
1.3.1.6 fumarate + NADH + H+ enzyme is required for optimal growth in presence of heme, enzyme plays a role in the generation of metabolic energy Bacteroides fragilis succinate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.6 FRD
-
 Bacteroides fragilis
1.3.1.6 fumarate reductase
-
 Bacteroides fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.6 7.6
-
 assay at Bacteroides fragilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.6 heme induces enzyme expression and is required for optimal anaerobic growth Bacteroides fragilis
1.3.1.6 NADH
-
 Bacteroides fragilis