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Literature summary extracted from

  • van der Geize, R.; Hessels, G.I.; Dijkhuizen, L.
    Molecular and functional characterization of the kstD2 gene of Rhodococcus erythropolis SQ1 encoding a second 3-ketosteroid DELTA(1)-dehydrogenase isoenzyme (2002), Microbiology, 148, 3285-3292.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.4 expression in Escherichia coli Rhodococcus erythropolis

Protein Variants

EC Number Protein Variants Comment Organism
1.3.99.4 S325F mutant enzyme has no detectable KSTD enzyme activity Rhodococcus erythropolis
1.3.99.4 T503I mutant enzyme has no detectable KSTD enzyme activity Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.4 0.017
-
 4-androstene-3,17-dione pH 7.0, 30°C Rhodococcus erythropolis
1.3.99.4 0.165
-
 9alpha-hydroxy-4-androstene-3,17-dione pH 7.0, 30°C Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + acceptor Rhodococcus erythropolis involved in steroid catabolism 1,4-androstadiene-3,17-dione + reduced acceptor
-
 ?
1.3.99.4 4-androstene-3,17-dione + acceptor Rhodococcus erythropolis SQ1 involved in steroid catabolism 1,4-androstadiene-3,17-dione + reduced acceptor
-
 ?
1.3.99.4 9alpha-hydroxy-4-androstene-3,17-dione + acceptor Rhodococcus erythropolis involved in steroid catabolism 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
 ?
1.3.99.4 9alpha-hydroxy-4-androstene-3,17-dione + acceptor Rhodococcus erythropolis SQ1 involved in steroid catabolism 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Rhodococcus erythropolis Q8RLT5
-
-
1.3.99.4 Rhodococcus erythropolis SQ1 Q8RLT5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + acceptor involved in steroid catabolism Rhodococcus erythropolis 1,4-androstadiene-3,17-dione + reduced acceptor
-
 ?
1.3.99.4 4-androstene-3,17-dione + acceptor acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium Rhodococcus erythropolis 1,4-androstadiene-3,17-dione + reduced acceptor
-
 ?
1.3.99.4 4-androstene-3,17-dione + acceptor involved in steroid catabolism Rhodococcus erythropolis SQ1 1,4-androstadiene-3,17-dione + reduced acceptor
-
 ?
1.3.99.4 4-androstene-3,17-dione + acceptor acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium Rhodococcus erythropolis SQ1 1,4-androstadiene-3,17-dione + reduced acceptor
-
 ?
1.3.99.4 9alpha-hydroxy-4-androstene-3,17-dione + acceptor involved in steroid catabolism Rhodococcus erythropolis 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
 ?
1.3.99.4 9alpha-hydroxy-4-androstene-3,17-dione + acceptor acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium Rhodococcus erythropolis 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
 ?
1.3.99.4 9alpha-hydroxy-4-androstene-3,17-dione + acceptor involved in steroid catabolism Rhodococcus erythropolis SQ1 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
 ?
1.3.99.4 9alpha-hydroxy-4-androstene-3,17-dione + acceptor acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium Rhodococcus erythropolis SQ1 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.99.4 ketosteroid DELTA1-dehydrogenase
-
 Rhodococcus erythropolis
1.3.99.4 KSTD2
-
 Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.4 7 8 30°C, reaction with 4-androstene-3,17-dione Rhodococcus erythropolis