BRENDA - Enzyme Database show

Metabolism of cyclic ADP-ribose: Zinc is an endogenous modulator of the cyclase/NAD glycohydrolase ratio of a CD38-like enzyme from human seminal fluid

Zielinska, W.; Barata, H.; Chini, E.N.; Life Sci. 74, 1781-1790 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.2.5
ATP
-
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
DTT
-
Homo sapiens
3.2.2.5
EDTA
inhibits the cyclase activity
Homo sapiens
3.2.2.5
nicotinamide
-
Homo sapiens
3.2.2.5
nicotinic acid
-
Homo sapiens
3.2.2.5
Zn2+
stimulates the cyclase activity, inhibits the NAD glycohydrolase activity, zinc has a regulatory function at physiological concentrations of 0.6-4 mM, responsible for modulation of the ADP-ribose cyclase/NAD glycohydrolase activity ratio of the bifunctional enzyme in zinc-rich seminal plasma to 1:3 in situ, with the purified enzyme in absence of zinc the ratio is 1:110-120, in presence of Zn2+ it is 1:2-3, mechanism
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.5
0.2
-
NAD+
pH 7.2, 37°C, purified enzyme
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.2.5
soluble
-
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.2.5
additional information
not affected by Cu2+, Mg2+, and Ca2+
Homo sapiens
3.2.2.5
Zinc
purified bifunctional enzyme has a ADP-ribosyl cyclase/NAD glycohydrolase ratio of 1/120. In situ cyclase/NAD glycohydrolase ratio measured in seminal plasma is 1/1. Physiological concentrations of zinc present in the seminal fluid, in the range of 0.6 to 4 mM, are responsible for the modulation of the cyclase/NAD glycohydrolase ratio
Homo sapiens
3.2.2.5
Zn2+
stimulates the cyclase activity, inhibits the NAD glycohydrolase activity, zinc has a regulatory function at physiological concentrations of 0.6-4 mM, responsible for modulation of the ADP-ribose cyclase/NAD glycohydrolase activity ratio of the bifunctional enzyme in zinc-rich seminal plasma to 1:3 in situ, with the purified enzyme in absence of zinc the ratio is 1:110-120, in presence of Zn2+ it is 1:2-3, mechanism
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.5
49000
-
x * 49000
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
additional information
Homo sapiens
bifunctional enzyme ADP-ribose cyclase/NAD glycohydrolase, enzyme activities are regulated by zinc, enzyme is capable of both synthesis and hydrolysis of cADP-ribose
?
-
-
-
3.2.2.5
NAD+ + H2O
Homo sapiens
-
ADP-ribose + nicotinamide
-
-
?
3.2.2.5
NAD+ + H2O
Homo sapiens
purified bifunctional enzyme has a ADP-ribosyl cyclase/NAD glycohydrolase ratio of 1/120. In situ cyclase/NAD glycohydrolase ratio measured in seminal plasma is 1/1. Physiological concentrations of zinc present in the seminal fluid, in the range of 0.6 to 4 mM, are responsible for the modulation of the cyclase/NAD glycohydrolase ratio
ADP-ribose + nicotinamide
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.5
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.2.5
from seminal plasma
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
seminal plasma
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
additional information
bifunctional enzyme ADP-ribose cyclase/NAD glycohydrolase, enzyme activities are regulated by zinc, enzyme is capable of both synthesis and hydrolysis of cADP-ribose
656684
Homo sapiens
?
-
-
-
-
3.2.2.5
additional information
bifunctional enzyme ADP-ribose cyclase/NAD glycohydrolase, enzyme is capable of both synthesis and hydrolysis of cADP-ribose
656684
Homo sapiens
?
-
-
-
-
3.2.2.5
NAD+ + H2O
-
656684
Homo sapiens
ADP-ribose + nicotinamide
-
-
-
?
3.2.2.5
NAD+ + H2O
purified bifunctional enzyme has a ADP-ribosyl cyclase/NAD glycohydrolase ratio of 1/120. In situ cyclase/NAD glycohydrolase ratio measured in seminal plasma is 1/1. Physiological concentrations of zinc present in the seminal fluid, in the range of 0.6 to 4 mM, are responsible for the modulation of the cyclase/NAD glycohydrolase ratio
656684
Homo sapiens
ADP-ribose + nicotinamide
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.2.5
?
x * 49000
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.2.5
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
6.6
-
purified enzyme
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.2.5
0.6
-
nicotinamide
pH 7.2, 37°C, purified enzyme
Homo sapiens
3.2.2.5
1.6
-
DTT
pH 7.2, 37°C, purified enzyme
Homo sapiens
3.2.2.5
9
-
nicotinic acid
pH 7.2, 37°C, purified enzyme
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.2.5
ATP
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
DTT
-
Homo sapiens
3.2.2.5
EDTA
inhibits the cyclase activity
Homo sapiens
3.2.2.5
nicotinamide
-
Homo sapiens
3.2.2.5
nicotinic acid
-
Homo sapiens
3.2.2.5
Zn2+
stimulates the cyclase activity, inhibits the NAD glycohydrolase activity, zinc has a regulatory function at physiological concentrations of 0.6-4 mM, responsible for modulation of the ADP-ribose cyclase/NAD glycohydrolase activity ratio of the bifunctional enzyme in zinc-rich seminal plasma to 1:3 in situ, with the purified enzyme in absence of zinc the ratio is 1:110-120, in presence of Zn2+ it is 1:2-3, mechanism
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.2.5
0.6
-
nicotinamide
pH 7.2, 37°C, purified enzyme
Homo sapiens
3.2.2.5
1.6
-
DTT
pH 7.2, 37°C, purified enzyme
Homo sapiens
3.2.2.5
9
-
nicotinic acid
pH 7.2, 37°C, purified enzyme
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.5
0.2
-
NAD+
pH 7.2, 37°C, purified enzyme
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.2.5
soluble
-
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.2.5
additional information
not affected by Cu2+, Mg2+, and Ca2+
Homo sapiens
3.2.2.5
Zinc
purified bifunctional enzyme has a ADP-ribosyl cyclase/NAD glycohydrolase ratio of 1/120. In situ cyclase/NAD glycohydrolase ratio measured in seminal plasma is 1/1. Physiological concentrations of zinc present in the seminal fluid, in the range of 0.6 to 4 mM, are responsible for the modulation of the cyclase/NAD glycohydrolase ratio
Homo sapiens
3.2.2.5
Zn2+
stimulates the cyclase activity, inhibits the NAD glycohydrolase activity, zinc has a regulatory function at physiological concentrations of 0.6-4 mM, responsible for modulation of the ADP-ribose cyclase/NAD glycohydrolase activity ratio of the bifunctional enzyme in zinc-rich seminal plasma to 1:3 in situ, with the purified enzyme in absence of zinc the ratio is 1:110-120, in presence of Zn2+ it is 1:2-3, mechanism
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.5
49000
-
x * 49000
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
additional information
Homo sapiens
bifunctional enzyme ADP-ribose cyclase/NAD glycohydrolase, enzyme activities are regulated by zinc, enzyme is capable of both synthesis and hydrolysis of cADP-ribose
?
-
-
-
3.2.2.5
NAD+ + H2O
Homo sapiens
-
ADP-ribose + nicotinamide
-
-
?
3.2.2.5
NAD+ + H2O
Homo sapiens
purified bifunctional enzyme has a ADP-ribosyl cyclase/NAD glycohydrolase ratio of 1/120. In situ cyclase/NAD glycohydrolase ratio measured in seminal plasma is 1/1. Physiological concentrations of zinc present in the seminal fluid, in the range of 0.6 to 4 mM, are responsible for the modulation of the cyclase/NAD glycohydrolase ratio
ADP-ribose + nicotinamide
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.5
from seminal plasma
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
seminal plasma
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
additional information
bifunctional enzyme ADP-ribose cyclase/NAD glycohydrolase, enzyme activities are regulated by zinc, enzyme is capable of both synthesis and hydrolysis of cADP-ribose
656684
Homo sapiens
?
-
-
-
-
3.2.2.5
additional information
bifunctional enzyme ADP-ribose cyclase/NAD glycohydrolase, enzyme is capable of both synthesis and hydrolysis of cADP-ribose
656684
Homo sapiens
?
-
-
-
-
3.2.2.5
NAD+ + H2O
-
656684
Homo sapiens
ADP-ribose + nicotinamide
-
-
-
?
3.2.2.5
NAD+ + H2O
purified bifunctional enzyme has a ADP-ribosyl cyclase/NAD glycohydrolase ratio of 1/120. In situ cyclase/NAD glycohydrolase ratio measured in seminal plasma is 1/1. Physiological concentrations of zinc present in the seminal fluid, in the range of 0.6 to 4 mM, are responsible for the modulation of the cyclase/NAD glycohydrolase ratio
656684
Homo sapiens
ADP-ribose + nicotinamide
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.2.5
?
x * 49000
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.2.5
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
6.6
-
purified enzyme
Homo sapiens