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Literature summary extracted from

  • Zielinska, W.; Barata, H.; Chini, E.N.
    Metabolism of cyclic ADP-ribose: Zinc is an endogenous modulator of the cyclase/NAD glycohydrolase ratio of a CD38-like enzyme from human seminal fluid (2004), Life Sci., 74, 1781-1790.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.5 ATP
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.5 DTT
-
 Homo sapiens
3.2.2.5 EDTA inhibits the cyclase activity Homo sapiens
3.2.2.5 nicotinamide
-
 Homo sapiens
3.2.2.5 nicotinic acid
-
 Homo sapiens
3.2.2.5 Zn2+ stimulates the cyclase activity, inhibits the NAD glycohydrolase activity, zinc has a regulatory function at physiological concentrations of 0.6-4 mM, responsible for modulation of the ADP-ribose cyclase/NAD glycohydrolase activity ratio of the bifunctional enzyme in zinc-rich seminal plasma to 1:3 in situ, with the purified enzyme in absence of zinc the ratio is 1:110-120, in presence of Zn2+ it is 1:2-3, mechanism Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.5 0.2
-
 NAD+ pH 7.2, 37°C, purified enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.5 soluble
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.5 additional information not affected by Cu2+, Mg2+, and Ca2+ Homo sapiens
3.2.2.5 Zinc purified bifunctional enzyme has a ADP-ribosyl cyclase/NAD glycohydrolase ratio of 1/120. In situ cyclase/NAD glycohydrolase ratio measured in seminal plasma is 1/1. Physiological concentrations of zinc present in the seminal fluid, in the range of 0.6 to 4 mM, are responsible for the modulation of the cyclase/NAD glycohydrolase ratio Homo sapiens
3.2.2.5 Zn2+ stimulates the cyclase activity, inhibits the NAD glycohydrolase activity, zinc has a regulatory function at physiological concentrations of 0.6-4 mM, responsible for modulation of the ADP-ribose cyclase/NAD glycohydrolase activity ratio of the bifunctional enzyme in zinc-rich seminal plasma to 1:3 in situ, with the purified enzyme in absence of zinc the ratio is 1:110-120, in presence of Zn2+ it is 1:2-3, mechanism Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.5 49000
-
 x * 49000 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.5 additional information Homo sapiens bifunctional enzyme ADP-ribose cyclase/NAD glycohydrolase, enzyme activities are regulated by zinc, enzyme is capable of both synthesis and hydrolysis of cADP-ribose ?
-
 ?
3.2.2.5 NAD+ + H2O Homo sapiens
-
 ADP-ribose + nicotinamide
-
 ?
3.2.2.5 NAD+ + H2O Homo sapiens purified bifunctional enzyme has a ADP-ribosyl cyclase/NAD glycohydrolase ratio of 1/120. In situ cyclase/NAD glycohydrolase ratio measured in seminal plasma is 1/1. Physiological concentrations of zinc present in the seminal fluid, in the range of 0.6 to 4 mM, are responsible for the modulation of the cyclase/NAD glycohydrolase ratio ADP-ribose + nicotinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.5 from seminal plasma Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.5 seminal plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.5 additional information bifunctional enzyme ADP-ribose cyclase/NAD glycohydrolase, enzyme activities are regulated by zinc, enzyme is capable of both synthesis and hydrolysis of cADP-ribose Homo sapiens ?
-
 ?
3.2.2.5 additional information bifunctional enzyme ADP-ribose cyclase/NAD glycohydrolase, enzyme is capable of both synthesis and hydrolysis of cADP-ribose Homo sapiens ?
-
 ?
3.2.2.5 NAD+ + H2O
-
 Homo sapiens ADP-ribose + nicotinamide
-
 ?
3.2.2.5 NAD+ + H2O purified bifunctional enzyme has a ADP-ribosyl cyclase/NAD glycohydrolase ratio of 1/120. In situ cyclase/NAD glycohydrolase ratio measured in seminal plasma is 1/1. Physiological concentrations of zinc present in the seminal fluid, in the range of 0.6 to 4 mM, are responsible for the modulation of the cyclase/NAD glycohydrolase ratio Homo sapiens ADP-ribose + nicotinamide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.5 ? x * 49000 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.2.5 CD38
-
 Homo sapiens
3.2.2.5 CD38 like activity
-
 Homo sapiens
3.2.2.5 NAD glycohydrolase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.5 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.5 6.6
-
 purified enzyme Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.2.5 0.6
-
 nicotinamide pH 7.2, 37°C, purified enzyme Homo sapiens
3.2.2.5 1.6
-
 DTT pH 7.2, 37°C, purified enzyme Homo sapiens
3.2.2.5 9
-
 nicotinic acid pH 7.2, 37°C, purified enzyme Homo sapiens