Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pfister, T.; Wimmer, E.
    Polypeptide p41 of a Norwalk-like virus is a nucleic acid-independent nucleoside triphosphatase (2001), J. Virol., 75, 1611-1619.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.15 CaCl2 in presence of Mn2+, 10 mM MgCl2 inhibits 20% Southampton virus
3.6.1.15 MgCl2 in presence of Mn2+, 10 mM MgCl2 inhibits 10% Southampton virus
3.6.1.15 NaCl 100 mM, inhibits ATP hydrolysis by 30% Southampton virus
3.6.1.15 polyA inhibits to a lesser extent than polyC and polyU Southampton virus
3.6.1.15 polyC 1 mg/l, 70% inhibition Southampton virus
3.6.1.15 polyG inhibits to a lesser extent than polyC and polyU Southampton virus
3.6.1.15 polyU 1 mg/l, 70% inhibition Southampton virus
3.6.1.15 RNA homopolymeric RNA inhibits under otherwise optimal conditions Southampton virus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.15 Mg2+ 20 mM, about 50% of the maximal activation obtained with Mn2+ Southampton virus
3.6.1.15 Mn2+ activates, 3.5-5 mM MnCl2 are optimal at an ATP concentration of 0.5 mM Southampton virus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.15 Southampton virus Q04544
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.15
-
 Southampton virus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.15 additional information viral protein is expressed in Escherichia coli cells Southampton virus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.15 ATP + H2O
-
 Southampton virus ADP + phosphate
-
 ?
3.6.1.15 CTP + H2O about 35% of activity with ATP Southampton virus CDP + phosphate
-
 ?
3.6.1.15 GTP + H2O about 90% of activity with ATP Southampton virus GDP + phosphate
-
 ?
3.6.1.15 additional information the enzyme does not possess helicase activity Southampton virus ?
-
 ?
3.6.1.15 UTP + H2O about 35% of activity with ATP Southampton virus UDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.15 nucleic acid-independent nucleoside triphosphatase
-
 Southampton virus
3.6.1.15 polypeptide p41
-
 Southampton virus
3.6.1.15 SHVp41
-
 Southampton virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.15 15
-
-
 Southampton virus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.15 1.4
-
 ATP
-
 Southampton virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.15 7.2 7.5 ATPase activity Southampton virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.15 6.8 8 pH 6.8: about 90% of maximal activity, pH 8.0: about 70% of maximal activity Southampton virus