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Literature summary extracted from
- Purification and characterization of 7beta-hydroxysteroid dehydrogenase from rabbit liver microsomes (2004), J. Steroid Biochem. Mol. Biol., 91, 185-190.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.201 | microsome | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.201 | 34000 | - |
x * 34000, SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.201 | 7beta-hydroxycholesterol + NADP+ | Oryctolagus cuniculus | - |
7-oxocholesterol + NADPH | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.201 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.201 | native enzyme from liver microsomes 300fold by ion exchange, hydroxylapatite, 2',5'-ADP affinity | Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.201 | liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.201 | 0.276 | - |
purified enzyme | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.201 | 5-cholesten-3beta,7beta-diol + NADP+ | i.e. 7beta-hydroxycholesterol | Oryctolagus cuniculus | 5-cholesten-3beta-ol-7-one + NADPH | - |
? | |
| 1.1.1.201 | 7beta-hydroxycholesterol + NADP+ | - |
Oryctolagus cuniculus | 7-oxocholesterol + NADPH | - |
? | |
| 1.1.1.201 | corticosterone + NADP+ | oxidation at C-11 | Oryctolagus cuniculus | 21-hydroxy-pregn-4-ene-3,11,20-trione + NADPH | - |
? | |
| 1.1.1.201 | cortisone + NADP+ | oxidation at C-11 | Oryctolagus cuniculus | ? + NADPH | - |
? | |
| 1.1.1.201 | additional information | enzyme might also show 11beta-hydroxysteroid dehydrogenase activity, no activity with cholesterol, 7alpha-hydroxycholesterol, 25-hydroxycholesterol, pregnenolone, dehydroepiandrosterone, chenodoxycolic acid, and ursodeoxycholic acid | Oryctolagus cuniculus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.201 | ? | x * 34000, SDS-PAGE | Oryctolagus cuniculus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.201 | 7beta-HSD | - |
Oryctolagus cuniculus |
| 1.1.1.201 | 7beta-hydroxysteroid dehydrogenase | - |
Oryctolagus cuniculus |
| 1.1.1.201 | More | enzyme might belong to the rabbit type I 11beta-HSD enzyme family | Oryctolagus cuniculus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.201 | 37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.201 | 7.7 | - |
potassium buffer | Oryctolagus cuniculus |
| 1.1.1.201 | 11 | - |
glycine-NaOH buffer | Oryctolagus cuniculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.201 | NADP+ | beta-form is preferred | Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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