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Literature summary extracted from

  • Ohno, S.; Matsumoto, N.; Watanabe, M.; Nakajin, S.
    Flavonoid inhibition of overexpressed human 3beta-hydroxysteroid dehydrogenase type II (2004), J. Steroid Biochem. Mol. Biol., 88, 175-182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 expression in Sf9 cells Homo sapiens
1.1.1.145 expression of the isozyme type II in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 6-hydroxyflavone 0.00056 mM, 50% inhibition; competitive Homo sapiens
1.1.1.145 apigenin 0.13 mM, 50% inhibition Homo sapiens
1.1.1.145 Biochanin A 0.00094 mM, 50% inhibition Homo sapiens
1.1.1.145 cyanoketone 0.0000076 mM, 50% inhibition; competitive Homo sapiens
1.1.1.145 daidzein 0.0033 mM, 50% inhibition Homo sapiens
1.1.1.145 flavanones diverse derivatives, inhibitory potential determination, overview Homo sapiens
1.1.1.145 flavones diverse derivatives, inhibitory potential determination, overview Homo sapiens
1.1.1.145 formononetin 0.0032 mM, 50% inhibition Homo sapiens
1.1.1.145 genistein 0.00026 mM, 50% inhibition; competitive Homo sapiens
1.1.1.145 isoflavones diverse derivatives, inhibitory potential determination, overview Homo sapiens
1.1.1.145 additional information structure-inhibition relationship Homo sapiens
1.1.1.145 myricetin 0.011 mM, 50% inhibition Homo sapiens
1.1.1.145 quercetin 0.084 mM, 50% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.0018
-
 dehydroepiandrosterone 37°C, pH 7.4 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+ Homo sapiens
-
 androst-5-en-3,17-dione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.145 a 3beta-hydroxy-DELTA5-steroid + NAD+ = a 3-oxo-DELTA5-steroid + NADH + H+ structure-function relationship Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Homo sapiens androst-5-en-3,17-dione + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 3beta-HSD type II
-
 Homo sapiens
1.1.1.145 3beta-hydroxysteroid dehydrogenase type II
-
 Homo sapiens
1.1.1.145 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4-isomerase type II
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.145 0.00000067
-
 cyanoketone recombinant enzyme Homo sapiens
1.1.1.145 0.00000067
-
 cyanoketone 37°C, pH 7.4 Homo sapiens
1.1.1.145 0.00012
-
 genistein recombinant enzyme Homo sapiens
1.1.1.145 0.00012
-
 genistein 37°C, pH 7.4 Homo sapiens
1.1.1.145 0.00019
-
 6-hydroxyflavone recombinant enzyme Homo sapiens
1.1.1.145 0.00019
-
 6-hydroxyflavone 37°C, pH 7.4 Homo sapiens