BRENDA - Enzyme Database show

Sterolsulphate sulphohydrolase from human placenta microsomes--30 kDa molecular weight form of cholesterol sulphate sulphohydrolase

Norkowska, M.; Gniot-Szulzycka, J.; J. Steroid Biochem. Mol. Biol. 81, 263-271 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.6.2
ascorbic acid
activates
Homo sapiens
3.1.6.2
Digitonin
activates
Homo sapiens
3.1.6.2
estrone
slightly activates
Homo sapiens
3.1.6.2
glutathione
slightly activates
Homo sapiens
3.1.6.2
additional information
not activated by 2-mercaptoethanol, cysteine or dithiothreitol
Homo sapiens
3.1.6.2
p-chloromercuribenzoate
activates
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.2
AgNO3
5 mM, 65% inhibition
Homo sapiens
3.1.6.2
cholesterol
1 mM, 57% inhibition
Homo sapiens
3.1.6.2
cysteine
10 mM, 15% inhibition
Homo sapiens
3.1.6.2
KH2PO4
5 mM, 25% inhibition
Homo sapiens
3.1.6.2
additional information
not inhibited by estrone, dehydroepiandrosterone, alkaline phosphatase treatment and phosphohydrolase inhibitors
Homo sapiens
3.1.6.2
Na2SO3
5 mM, 43% inhibition
Homo sapiens
3.1.6.2
Na2SO4
5 mM, 18% inhibition
Homo sapiens
3.1.6.2
pregnenolone
1 mM, 10% inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.0067
-
Cholesterol sulfate
pH 6.4, 37°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.2
microsome
placental, at least two distinct forms of CHS-ase
Homo sapiens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.2
30000
-
x * 30000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
cholesterol sulfate + H2O
Homo sapiens
cholesterol sulfate metabolism, steroid sulfohydrolase pathway
cholesterol + sulfate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.2
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.6.2
solubilized with 0.5% Triton X-100, two fractions with cholesterol sulfate sulfohydrolase activity are separated by DEAE-cellulose chromatography, purification of the 30 kDa enzyme
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
placenta
microsomes, at least two distinct forms of CHS-ase
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.6.2
0.385
-
pH 6.4, 37°C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
cholesterol sulfate + H2O
cholesterol sulfate metabolism, steroid sulfohydrolase pathway
656643
Homo sapiens
cholesterol + sulfate
-
-
-
?
3.1.6.2
cholesterol sulfate + H2O
highly specific for cholesterol sulfate
656643
Homo sapiens
cholesterol + sulfate
-
-
-
?
3.1.6.2
dehydroepiandrosterone sulfate + H2O
1.5% of the activity with cholesterol sulfate
656643
Homo sapiens
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
0.6% of the activity with cholesterol sulfate
656643
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
pregnenolone sulfate + H2O
8.7% of the activity with cholesterol sulfate
656643
Homo sapiens
pregnenolone + sulfate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.6.2
?
x * 30000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.2
30
-
-
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.6.2
60
-
5 min, 40% loss of activity
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
6.4
-
-
Homo sapiens
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.6.2
Homo sapiens
isoelectric focussing, pH-gradient 2-10
-
7.1
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.6.2
ascorbic acid
activates
Homo sapiens
3.1.6.2
Digitonin
activates
Homo sapiens
3.1.6.2
estrone
slightly activates
Homo sapiens
3.1.6.2
glutathione
slightly activates
Homo sapiens
3.1.6.2
additional information
not activated by 2-mercaptoethanol, cysteine or dithiothreitol
Homo sapiens
3.1.6.2
p-chloromercuribenzoate
activates
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.2
AgNO3
5 mM, 65% inhibition
Homo sapiens
3.1.6.2
cholesterol
1 mM, 57% inhibition
Homo sapiens
3.1.6.2
cysteine
10 mM, 15% inhibition
Homo sapiens
3.1.6.2
KH2PO4
5 mM, 25% inhibition
Homo sapiens
3.1.6.2
additional information
not inhibited by estrone, dehydroepiandrosterone, alkaline phosphatase treatment and phosphohydrolase inhibitors
Homo sapiens
3.1.6.2
Na2SO3
5 mM, 43% inhibition
Homo sapiens
3.1.6.2
Na2SO4
5 mM, 18% inhibition
Homo sapiens
3.1.6.2
pregnenolone
1 mM, 10% inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.0067
-
Cholesterol sulfate
pH 6.4, 37°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.2
microsome
placental, at least two distinct forms of CHS-ase
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.2
30000
-
x * 30000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
cholesterol sulfate + H2O
Homo sapiens
cholesterol sulfate metabolism, steroid sulfohydrolase pathway
cholesterol + sulfate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.2
solubilized with 0.5% Triton X-100, two fractions with cholesterol sulfate sulfohydrolase activity are separated by DEAE-cellulose chromatography, purification of the 30 kDa enzyme
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
placenta
microsomes, at least two distinct forms of CHS-ase
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.6.2
0.385
-
pH 6.4, 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
cholesterol sulfate + H2O
cholesterol sulfate metabolism, steroid sulfohydrolase pathway
656643
Homo sapiens
cholesterol + sulfate
-
-
-
?
3.1.6.2
cholesterol sulfate + H2O
highly specific for cholesterol sulfate
656643
Homo sapiens
cholesterol + sulfate
-
-
-
?
3.1.6.2
dehydroepiandrosterone sulfate + H2O
1.5% of the activity with cholesterol sulfate
656643
Homo sapiens
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
0.6% of the activity with cholesterol sulfate
656643
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
pregnenolone sulfate + H2O
8.7% of the activity with cholesterol sulfate
656643
Homo sapiens
pregnenolone + sulfate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.6.2
?
x * 30000, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.2
30
-
-
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.6.2
60
-
5 min, 40% loss of activity
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
6.4
-
-
Homo sapiens
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.6.2
Homo sapiens
isoelectric focussing, pH-gradient 2-10
-
7.1