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Literature summary extracted from
- Sterolsulphate sulphohydrolase from human placenta microsomes--30 kDa molecular weight form of cholesterol sulphate sulphohydrolase (2002), J. Steroid Biochem. Mol. Biol., 81, 263-271.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.2 | ascorbic acid | activates | Homo sapiens |  |
| 3.1.6.2 | Digitonin | activates | Homo sapiens | |
| 3.1.6.2 | estrone | slightly activates | Homo sapiens | |
| 3.1.6.2 | glutathione | slightly activates | Homo sapiens | |
| 3.1.6.2 | additional information | not activated by 2-mercaptoethanol, cysteine or dithiothreitol | Homo sapiens | |
| 3.1.6.2 | p-chloromercuribenzoate | activates | Homo sapiens | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.2 | AgNO3 | 5 mM, 65% inhibition | Homo sapiens | |
| 3.1.6.2 | cholesterol | 1 mM, 57% inhibition | Homo sapiens | |
| 3.1.6.2 | cysteine | 10 mM, 15% inhibition | Homo sapiens | |
| 3.1.6.2 | KH2PO4 | 5 mM, 25% inhibition | Homo sapiens | |
| 3.1.6.2 | additional information | not inhibited by estrone, dehydroepiandrosterone, alkaline phosphatase treatment and phosphohydrolase inhibitors | Homo sapiens | |
| 3.1.6.2 | Na2SO3 | 5 mM, 43% inhibition | Homo sapiens | |
| 3.1.6.2 | Na2SO4 | 5 mM, 18% inhibition | Homo sapiens | |
| 3.1.6.2 | pregnenolone | 1 mM, 10% inhibition | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.2 | 0.0067 | - |
Cholesterol sulfate | pH 6.4, 37°C | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.6.2 | microsome | placental, at least two distinct forms of CHS-ase | Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 30000 | - |
x * 30000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.2 | cholesterol sulfate + H2O | Homo sapiens | cholesterol sulfate metabolism, steroid sulfohydrolase pathway | cholesterol + sulfate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.2 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.2 | solubilized with 0.5% Triton X-100, two fractions with cholesterol sulfate sulfohydrolase activity are separated by DEAE-cellulose chromatography, purification of the 30 kDa enzyme | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.6.2 | placenta | microsomes, at least two distinct forms of CHS-ase | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 0.385 | - |
pH 6.4, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.2 | cholesterol sulfate + H2O | cholesterol sulfate metabolism, steroid sulfohydrolase pathway | Homo sapiens | cholesterol + sulfate | - |
? | |
| 3.1.6.2 | cholesterol sulfate + H2O | highly specific for cholesterol sulfate | Homo sapiens | cholesterol + sulfate | - |
? | |
| 3.1.6.2 | dehydroepiandrosterone sulfate + H2O | 1.5% of the activity with cholesterol sulfate | Homo sapiens | dehydroepiandrosterone + sulfate | - |
? | |
| 3.1.6.2 | estrone sulfate + H2O | 0.6% of the activity with cholesterol sulfate | Homo sapiens | estrone + sulfate | - |
? | |
| 3.1.6.2 | pregnenolone sulfate + H2O | 8.7% of the activity with cholesterol sulfate | Homo sapiens | pregnenolone + sulfate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.6.2 | ? | x * 30000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.6.2 | cholesterol sulfate sulfohydrolase | - |
Homo sapiens |
| 3.1.6.2 | CHS-ase | - |
Homo sapiens |
| 3.1.6.2 | sterolsulfate sulfohydrolase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 30 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 60 | - |
5 min, 40% loss of activity | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 6.4 | - |
- |
Homo sapiens |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.6.2 | Homo sapiens | isoelectric focussing, pH-gradient 2-10 | - |
7.1 |
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