BRENDA - Enzyme Database show

Purification, characterization and crystallization of human placental estrone/dehydroepiandrosterone sulfatase, a membrane-bound enzyme of the endoplasmic reticulum

Hernandez-Guzman, F.G.; Higashiyama, T.; Osawa, Y.; Ghosh, D.; J. Steroid Biochem. Mol. Biol. 78, 441-450 (2001)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.1.6.2
medicine
X-linked ichthyosis is ascribed to the non-functional ES resulting from point and/or deletion mutations in the ES gene
Homo sapiens
3.1.6.2
pharmacology
enzyme inhibitors can be used as anti-tumor agents
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.1.6.2
full-length, active enzyme form, X-ray analysis
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.00959
-
dehydroepiandrosterone sulfate
pH 8, 37°C
Homo sapiens
3.1.6.2
0.07275
-
Estrone sulfate
pH 8, 37°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.2
endoplasmic reticulum
membrane-bound
Homo sapiens
5783
-
3.1.6.2
membrane
bound to the membrane of the endoplasmic reticulum
Homo sapiens
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.2
additional information
-
native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states
Homo sapiens
3.1.6.2
65000
-
x * 65000, SDS-PAGE
Homo sapiens
3.1.6.2
250000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
additional information
Homo sapiens
ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.2
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.6.2
glycoprotein
N-linked glycosylation at two of the possible four sites
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
3.1.6.2
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.6.2
additional information
-
-
Homo sapiens
3.1.6.2
1.556
-
pH 8, 37°C, hydrolysis of dehydroepiandrosterone sulfate
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.1.6.2
room temperature, crystalline state of enzyme, more than 30 days, less than 33% loss of specific activity
Homo sapiens
3.1.6.2
room temperature, n-octyl-beta-D-glucopyranoside, several weeks, nearly stable
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
dehydroepiandrosterone sulfate + H2O
-
656641
Homo sapiens
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
656641
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
additional information
ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors
656641
Homo sapiens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.1.6.2
?
x * 65000, SDS-PAGE
Homo sapiens
3.1.6.2
More
all oligomeric states between a monomer and a tetramer are possible, varying aggregation properties of enzyme under various buffer, pH, detergent and ionic strength conditions, gel filtration suggests ES to be predominantly a homotetramer, native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.2
37
-
assay at
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.6.2
6.48
-
dehydroepiandrosterone sulfate
pH 8, 37°C
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
8
-
assay at
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.6.2
medicine
X-linked ichthyosis is ascribed to the non-functional ES resulting from point and/or deletion mutations in the ES gene
Homo sapiens
3.1.6.2
pharmacology
enzyme inhibitors can be used as anti-tumor agents
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.1.6.2
full-length, active enzyme form, X-ray analysis
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.00959
-
dehydroepiandrosterone sulfate
pH 8, 37°C
Homo sapiens
3.1.6.2
0.07275
-
Estrone sulfate
pH 8, 37°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.2
endoplasmic reticulum
membrane-bound
Homo sapiens
5783
-
3.1.6.2
membrane
bound to the membrane of the endoplasmic reticulum
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.2
additional information
-
native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states
Homo sapiens
3.1.6.2
65000
-
x * 65000, SDS-PAGE
Homo sapiens
3.1.6.2
250000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
additional information
Homo sapiens
ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors
?
-
-
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.6.2
glycoprotein
N-linked glycosylation at two of the possible four sites
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.2
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.6.2
additional information
-
-
Homo sapiens
3.1.6.2
1.556
-
pH 8, 37°C, hydrolysis of dehydroepiandrosterone sulfate
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.6.2
room temperature, crystalline state of enzyme, more than 30 days, less than 33% loss of specific activity
Homo sapiens
3.1.6.2
room temperature, n-octyl-beta-D-glucopyranoside, several weeks, nearly stable
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
dehydroepiandrosterone sulfate + H2O
-
656641
Homo sapiens
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
656641
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
additional information
ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors
656641
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.6.2
?
x * 65000, SDS-PAGE
Homo sapiens
3.1.6.2
More
all oligomeric states between a monomer and a tetramer are possible, varying aggregation properties of enzyme under various buffer, pH, detergent and ionic strength conditions, gel filtration suggests ES to be predominantly a homotetramer, native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.2
37
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.6.2
6.48
-
dehydroepiandrosterone sulfate
pH 8, 37°C
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
8
-
assay at
Homo sapiens