Any feedback?
Literature summary extracted from
- Purification, characterization and crystallization of human placental estrone/dehydroepiandrosterone sulfatase, a membrane-bound enzyme of the endoplasmic reticulum (2001), J. Steroid Biochem. Mol. Biol., 78, 441-450.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.6.2 | medicine | X-linked ichthyosis is ascribed to the non-functional ES resulting from point and/or deletion mutations in the ES gene | Homo sapiens |
| 3.1.6.2 | pharmacology | enzyme inhibitors can be used as anti-tumor agents | Homo sapiens |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.6.2 | full-length, active enzyme form, X-ray analysis | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.2 | 0.00959 | - |
dehydroepiandrosterone sulfate | pH 8, 37°C | Homo sapiens |  |
| 3.1.6.2 | 0.07275 | - |
Estrone sulfate | pH 8, 37°C | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.6.2 | endoplasmic reticulum | membrane-bound | Homo sapiens | 5783 | - |
| 3.1.6.2 | membrane | bound to the membrane of the endoplasmic reticulum | Homo sapiens | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | additional information | - |
native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states | Homo sapiens |
| 3.1.6.2 | 65000 | - |
x * 65000, SDS-PAGE | Homo sapiens |
| 3.1.6.2 | 250000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.2 | additional information | Homo sapiens | ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.2 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.6.2 | glycoprotein | N-linked glycosylation at two of the possible four sites | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.2 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.6.2 | placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | additional information | - |
- |
Homo sapiens |
| 3.1.6.2 | 1.556 | - |
pH 8, 37°C, hydrolysis of dehydroepiandrosterone sulfate | Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.6.2 | room temperature, crystalline state of enzyme, more than 30 days, less than 33% loss of specific activity | Homo sapiens |
| 3.1.6.2 | room temperature, n-octyl-beta-D-glucopyranoside, several weeks, nearly stable | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.2 | dehydroepiandrosterone sulfate + H2O | - |
Homo sapiens | dehydroepiandrosterone + sulfate | - |
? | |
| 3.1.6.2 | estrone sulfate + H2O | - |
Homo sapiens | estrone + sulfate | - |
? | |
| 3.1.6.2 | additional information | ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.6.2 | ? | x * 65000, SDS-PAGE | Homo sapiens |
| 3.1.6.2 | More | all oligomeric states between a monomer and a tetramer are possible, varying aggregation properties of enzyme under various buffer, pH, detergent and ionic strength conditions, gel filtration suggests ES to be predominantly a homotetramer, native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.6.2 | DHEAS | - |
Homo sapiens |
| 3.1.6.2 | ES | - |
Homo sapiens |
| 3.1.6.2 | estrone sulfatase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.2 | 6.48 | - |
dehydroepiandrosterone sulfate | pH 8, 37°C | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.2 | 8 | - |
assay at | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
