Literature summary extracted from

Park, I.H.; Han, B.K.; Baek, J.H.; Ryu, Y.W.; Park, Y.H.; Jo, D.H.
Subunits of neurosteroid sulfatase from bovine brain (2000), J. Steroid Biochem. Mol. Biol., 73, 135-139.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.1.6.2	Triton X-100	activates	Bos taurus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.1.6.2	microsome	-	Bos taurus	-	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.6.2	38000	-	alpha2,beta2, 2 * 57000 + 2 * 38000, two 57 kDa catalytic and two 38 kDa regulatory subunits, SDS-PAGE	Bos taurus
3.1.6.2	57000	-	alpha2,beta2, 2 * 57000 + 2 * 38000, two 57 kDa catalytic and two 38 kDa regulatory subunits, SDS-PAGE	Bos taurus
3.1.6.2	180000	-	gel filtration, in the presence of Triton X-100	Bos taurus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.1.6.2	additional information	Bos taurus	NSS likely plays a key role in the regulation of the GABA-A receptor	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.6.2	Bos taurus	-	2-2.5 years old females	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.6.2	99.3fold	Bos taurus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.6.2	brain	-	Bos taurus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.6.2	0.000002961	-	-	Bos taurus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.6.2	dehydroepiandrosterone sulfate + H2O	-	Bos taurus	dehydroepiandrosterone + sulfate	-	?
3.1.6.2	additional information	NSS likely plays a key role in the regulation of the GABA-A receptor	Bos taurus	?	-	?
3.1.6.2	pregnenolone sulfate + H2O	-	Bos taurus	pregnenolone + sulfate	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.1.6.2	heterotetramer	alpha2,beta2, 2 * 57000 + 2 * 38000, two 57 kDa catalytic and two 38 kDa regulatory subunits, SDS-PAGE	Bos taurus

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.6.2	neurosteroid sulfatase	-	Bos taurus
3.1.6.2	NSS	-	Bos taurus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.1.6.2	37	-	assay at	Bos taurus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.1.6.2	7.4	-	assay at	Bos taurus

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Release 2023.1 (January 2023)