BRENDA - Enzyme Database

Cytosolic and chloroplastic NADP-dependent isocitrate dehydrogenases in Spirodela polyrhiza. I. Regulation of activity by metabolites in vitro

Popova, T.N.; Rakhmanova, T.I.; Appenroth, K.J.; J. Plant Physiol. 159, 231-237 (2002)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.42
cis-aconitate
isozyme ICDH2, not isozyme ICDH1
Spirodela polyrhiza
1.1.1.42
citrate
isozymes ICDH2 and ICDH1
Spirodela polyrhiza
1.1.1.42
glutamate
160% stimulation of isozyme ICDH1 at 0.3 mM
Spirodela polyrhiza
1.1.1.42
isocitrate
isozymes ICDH2 and ICDH1
Spirodela polyrhiza
1.1.1.42
additional information
no effect by trans-aconitate on isozyme ICDH2
Spirodela polyrhiza
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
isozymes ICDH2 and ICDH1, isozyme ICDH2 is more sensitive to negative feedback inhibition by 2-oxoglutarate
Spirodela polyrhiza
1.1.1.42
cis-aconitate
isozyme ICDH1, not isozyme ICDH2
Spirodela polyrhiza
1.1.1.42
glutamate
80% inhibition of isozyme ICDH2 at 2 mM
Spirodela polyrhiza
1.1.1.42
succinate
isozymes ICDH2 and ICDH1
Spirodela polyrhiza
1.1.1.42
trans-aconitate
isozyme ICDH1, not isozyme ICDH2
Spirodela polyrhiza
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.07
-
isocitrate
chloroplastic isozyme ICDH2, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.08
-
Mn2+
chloroplastic isozyme ICDH2, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.08
-
NADP+
chloroplastic isozyme ICDH2, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.12
-
Mg2+
chloroplastic isozyme ICDH2, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.17
-
NADP+
cytosolic isozyme ICDH1, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.18
-
isocitrate
cytosolic isozyme ICDH1, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.21
-
Mn2+
cytosolic isozyme ICDH1, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.31
-
Mg2+
cytosolic isozyme ICDH1, pH 7.8, 25C
Spirodela polyrhiza
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
chloroplast
isozyme ICDH2, minor form
Spirodela polyrhiza
9507
-
1.1.1.42
cytosol
isozyme ICDH1, major form
Spirodela polyrhiza
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
-
Spirodela polyrhiza
1.1.1.42
Mn2+
-
Spirodela polyrhiza
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Spirodela polyrhiza
metabolic regulation of the isozymes
2-oxoglutarate + CO2 + NADPH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Spirodela polyrhiza
-
duck weed, isozymes ICDH1 and ICDH2
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
isozymes ICDH1, 172fold, and ICDH2, 31fold, by ammonium sulfate fractionation, desalting, DEAE ion exchange and gel filtration
Spirodela polyrhiza
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.42
0.0046
-
purified chloroplastic isozyme ICDH1
Spirodela polyrhiza
1.1.1.42
0.038
-
purified cytosolic isozyme ICDH1
Spirodela polyrhiza
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
656616
Spirodela polyrhiza
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
metabolic regulation of the isozymes
656616
Spirodela polyrhiza
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
25
-
assay at
Spirodela polyrhiza
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.8
7.9
isozymes ICDH2 and ICDH1
Spirodela polyrhiza
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Spirodela polyrhiza
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.42
cis-aconitate
isozyme ICDH2, not isozyme ICDH1
Spirodela polyrhiza
1.1.1.42
citrate
isozymes ICDH2 and ICDH1
Spirodela polyrhiza
1.1.1.42
glutamate
160% stimulation of isozyme ICDH1 at 0.3 mM
Spirodela polyrhiza
1.1.1.42
isocitrate
isozymes ICDH2 and ICDH1
Spirodela polyrhiza
1.1.1.42
additional information
no effect by trans-aconitate on isozyme ICDH2
Spirodela polyrhiza
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Spirodela polyrhiza
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
isozymes ICDH2 and ICDH1, isozyme ICDH2 is more sensitive to negative feedback inhibition by 2-oxoglutarate
Spirodela polyrhiza
1.1.1.42
cis-aconitate
isozyme ICDH1, not isozyme ICDH2
Spirodela polyrhiza
1.1.1.42
glutamate
80% inhibition of isozyme ICDH2 at 2 mM
Spirodela polyrhiza
1.1.1.42
succinate
isozymes ICDH2 and ICDH1
Spirodela polyrhiza
1.1.1.42
trans-aconitate
isozyme ICDH1, not isozyme ICDH2
Spirodela polyrhiza
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.07
-
isocitrate
chloroplastic isozyme ICDH2, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.08
-
Mn2+
chloroplastic isozyme ICDH2, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.08
-
NADP+
chloroplastic isozyme ICDH2, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.12
-
Mg2+
chloroplastic isozyme ICDH2, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.17
-
NADP+
cytosolic isozyme ICDH1, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.18
-
isocitrate
cytosolic isozyme ICDH1, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.21
-
Mn2+
cytosolic isozyme ICDH1, pH 7.8, 25C
Spirodela polyrhiza
1.1.1.42
0.31
-
Mg2+
cytosolic isozyme ICDH1, pH 7.8, 25C
Spirodela polyrhiza
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
chloroplast
isozyme ICDH2, minor form
Spirodela polyrhiza
9507
-
1.1.1.42
cytosol
isozyme ICDH1, major form
Spirodela polyrhiza
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
-
Spirodela polyrhiza
1.1.1.42
Mn2+
-
Spirodela polyrhiza
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Spirodela polyrhiza
metabolic regulation of the isozymes
2-oxoglutarate + CO2 + NADPH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
isozymes ICDH1, 172fold, and ICDH2, 31fold, by ammonium sulfate fractionation, desalting, DEAE ion exchange and gel filtration
Spirodela polyrhiza
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.42
0.0046
-
purified chloroplastic isozyme ICDH1
Spirodela polyrhiza
1.1.1.42
0.038
-
purified cytosolic isozyme ICDH1
Spirodela polyrhiza
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
656616
Spirodela polyrhiza
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
metabolic regulation of the isozymes
656616
Spirodela polyrhiza
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
25
-
assay at
Spirodela polyrhiza
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.8
7.9
isozymes ICDH2 and ICDH1
Spirodela polyrhiza