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Literature summary extracted from

  • Popova, T.N.; Rakhmanova, T.I.; Appenroth, K.J.
    Cytosolic and chloroplastic NADP-dependent isocitrate dehydrogenases in Spirodela polyrhiza. I. Regulation of activity by metabolites in vitro (2002), J. Plant Physiol., 159, 231-237.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.42 cis-aconitate isozyme ICDH2, not isozyme ICDH1 Spirodela polyrhiza
1.1.1.42 citrate isozymes ICDH2 and ICDH1 Spirodela polyrhiza
1.1.1.42 glutamate 160% stimulation of isozyme ICDH1 at 0.3 mM Spirodela polyrhiza
1.1.1.42 isocitrate isozymes ICDH2 and ICDH1 Spirodela polyrhiza
1.1.1.42 additional information no effect by trans-aconitate on isozyme ICDH2 Spirodela polyrhiza

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 2-oxoglutarate isozymes ICDH2 and ICDH1, isozyme ICDH2 is more sensitive to negative feedback inhibition by 2-oxoglutarate Spirodela polyrhiza
1.1.1.42 cis-aconitate isozyme ICDH1, not isozyme ICDH2 Spirodela polyrhiza
1.1.1.42 glutamate 80% inhibition of isozyme ICDH2 at 2 mM Spirodela polyrhiza
1.1.1.42 succinate isozymes ICDH2 and ICDH1 Spirodela polyrhiza
1.1.1.42 trans-aconitate isozyme ICDH1, not isozyme ICDH2 Spirodela polyrhiza

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.07
-
 isocitrate chloroplastic isozyme ICDH2, pH 7.8, 25°C Spirodela polyrhiza
1.1.1.42 0.08
-
 NADP+ chloroplastic isozyme ICDH2, pH 7.8, 25°C Spirodela polyrhiza
1.1.1.42 0.08
-
 Mn2+ chloroplastic isozyme ICDH2, pH 7.8, 25°C Spirodela polyrhiza
1.1.1.42 0.12
-
 Mg2+ chloroplastic isozyme ICDH2, pH 7.8, 25°C Spirodela polyrhiza
1.1.1.42 0.17
-
 NADP+ cytosolic isozyme ICDH1, pH 7.8, 25°C Spirodela polyrhiza
1.1.1.42 0.18
-
 isocitrate cytosolic isozyme ICDH1, pH 7.8, 25°C Spirodela polyrhiza
1.1.1.42 0.21
-
 Mn2+ cytosolic isozyme ICDH1, pH 7.8, 25°C Spirodela polyrhiza
1.1.1.42 0.31
-
 Mg2+ cytosolic isozyme ICDH1, pH 7.8, 25°C Spirodela polyrhiza

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 chloroplast isozyme ICDH2, minor form Spirodela polyrhiza 9507
-
1.1.1.42 cytosol isozyme ICDH1, major form Spirodela polyrhiza 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+
-
 Spirodela polyrhiza
1.1.1.42 Mn2+
-
 Spirodela polyrhiza

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Spirodela polyrhiza metabolic regulation of the isozymes 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Spirodela polyrhiza
-
 duck weed, isozymes ICDH1 and ICDH2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 isozymes ICDH1, 172fold, and ICDH2, 31fold, by ammonium sulfate fractionation, desalting, DEAE ion exchange and gel filtration Spirodela polyrhiza

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 0.0046
-
 purified chloroplastic isozyme ICDH1 Spirodela polyrhiza
1.1.1.42 0.038
-
 purified cytosolic isozyme ICDH1 Spirodela polyrhiza

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Spirodela polyrhiza 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 isocitrate + NADP+ metabolic regulation of the isozymes Spirodela polyrhiza 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 ICDH
-
 Spirodela polyrhiza
1.1.1.42 NADP-dependent isocitrate dehydrogenase
-
 Spirodela polyrhiza

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 25
-
 assay at Spirodela polyrhiza

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.8 7.9 isozymes ICDH2 and ICDH1 Spirodela polyrhiza

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Spirodela polyrhiza