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Literature summary extracted from

  • Strumilo, S.; Czygier, M.; Kondracikowska, J.; Dobrzyn, P.; Czerniecki, J.
    Kinetic and spectral investigation of allosteric interaction of coenzymes with 2-oxo acid dehydrogenase complexes (2002), J. Mol. Struct., 614, 221-226.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.104 thiamine diphosphate exogenous addition activates, involved in conformational changes Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.104 additional information
-
 additional information kinetic study, reaction of pyruvate dehydrogenase complex Bos taurus
1.2.1.104 0.0002
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate, in presence of 2 mM Mn2+ Bos taurus
1.2.1.104 0.0006
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate, in presence of 2 mM Ca2+ Bos taurus
1.2.1.104 0.0007
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate, in presence of 2 mM Mn2+ Bos taurus
1.2.1.104 0.0016
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate, in presence of 2 mM Ca2+ Bos taurus
1.2.1.104 0.004
-
 CoA 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate Bos taurus
1.2.1.104 0.012
-
 CoA 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate Bos taurus
1.2.1.104 0.023
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate, without divalent cations Bos taurus
1.2.1.104 0.033
-
 NAD+ 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate Bos taurus
1.2.1.104 0.063
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate and divalent cations Bos taurus
1.2.1.104 0.07
-
 NAD+ 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate Bos taurus
1.2.1.105 additional information
-
 additional information kinetic study, reaction of 2-oxoglutarate dehydrogenase complex Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.105 Ca2+ activates the 2-oxoglutarate dehydrogenase complex activity Bos taurus
1.2.1.105 Mn2+ activates the 2-oxoglutarate dehydrogenase complex activity Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.104 pyruvate + CoA + NAD+ Bos taurus
-
 acetyl-CoA + CO2 + NADH
-
 ir
1.2.1.105 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine Bos taurus
-
 [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Bos taurus
-
 pyruvate dehydrogenase complex
-
1.2.1.105 Bos taurus
-
 2-oxoglutarate dehydrogenase complex
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.104 pyruvate dehydrogenase complex from ox heart, by PEG fractionation, ultracentrifugation, and gel filtration Bos taurus
1.2.1.105 2-oxoglutarate dehydrogenase complex from ox heart, by PEG fractionation, ultracentrifugation, and gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.104 heart
-
 Bos taurus
-
1.2.1.104 myocardium
-
 Bos taurus
-
1.2.1.105 heart
-
 Bos taurus
-
1.2.1.105 myocardium
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.104 pyruvate + CoA + NAD+
-
 Bos taurus acetyl-CoA + CO2 + NADH
-
 ir
1.2.1.105 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine
-
 Bos taurus [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.2.1.104 PDC
-
 Bos taurus
1.2.1.104 pyruvate dehydrogenase complex
-
 Bos taurus
1.2.1.105 2-oxoglutarate dehydrogenase complex
-
 Bos taurus
1.2.1.105 OGDC
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.104 30
-
 assay at Bos taurus
1.2.1.105 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.104 7.6
-
 assay at Bos taurus
1.2.1.105 7.6
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.104 NAD+
-
 Bos taurus
1.2.1.105 NAD+
-
 Bos taurus
1.2.1.105 thiamine diphosphate activates the 2-oxoglutarate dehydrogenase complex activity, involved in conformational changes Bos taurus